The Effect of Hyperthermia on Survival Fraction of DU 145 Human Prostate Carcinoma Cell Line in Monolayer and Spheroid Culture

Abstract

Background: Hyperthermia (also called thermal therapy or thermotherapy) is a type of cancer treatment in which body tissue is exposed to high temperatures (up to 113°F). Research has shown that high temperatures can damage and kill cancer cells, usually with minimal injury to normal tissues. For many years, biologists have investigated cancer by using monolayer cell culture. It is becoming more and more apparent that two-dimensional cell culture (monolayer) can not completely represent the real structure and characteristics of three-dimensional in vivo solid tumors. These spheroids resemble in vivo tumor models in several aspects. Therefore, studying growth characteristics and behavior of spheroids is beneficial in understanding the behavior of tumors under various experimental conditions.