Effect of Extracts of <i>Capparis spinosa</i>, <i>Withania somnifera</i> and <i>Peganum harmala</i> on <i>Aspergillus flavus</i> Growth and Expression of Major Genes in Aflatoxin Biosynthetic Pathway
| Author | Ahmad Mehraban | en |
| Author | Javad Abkhoo | en |
| Author | Ebrahim Dahmardeh | en |
| Issued Date | 2018-05-31 | en |
| Abstract | Background: Aflatoxins, a polyketide group derived from furanocoumarins, are highly toxic carcinogens produced by Aspergillus flavus and other closely related species. Objectives: In this research, the effects of Capparis spinosa, Withania somnifera, and Peganum harmala extracts on A. flavus growth and the expression of some major genes in aflatoxin biosynthetic pathway were investigated. Methods: Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were measured and the expression of aflQ, aflO, aflS, and aflR genes was evaluated by using quantitative real-time polymerase chain reaction (qRT-PCR) technique. Results: Capparis spinosa extract had the lowest MIC value (200 mg/mL) and the extracts of W. somnifera and P. harmala had the highest MIC value (400 mg/mL). The extracts of W. somnifera and P. harmala had no fungicidal activity, and the C. spinosa extract had the lowest MFC value (400 mg/mL) and had the highest fungicidal effect on A. flavus. The expression of aflQ, aflO, aflS, and aflR genes was significantly decreased by the C. spinosa extract. Conclusions: In general, the C. spinosa extract could have fungicidal and inhibitory effects on A. flavus and reduce expression of major genes in the aflatoxin biosynthetic pathway. | en |
| DOI | https://doi.org/10.5812/zjrms.64602 | en |
| Keyword | Antifungal Activity | en |
| Keyword | Aflatoxin | en |
| Keyword | <i>Aspergillus flavus</i> | en |
| Keyword | <i>Capparis spinosa</i> | en |
| Keyword | <i>Withania somnifera</i> | en |
| Keyword | <i>Peganum harmala</i> | en |
| Publisher | Brieflands | en |
| Title | Effect of Extracts of <i>Capparis spinosa</i>, <i>Withania somnifera</i> and <i>Peganum harmala</i> on <i>Aspergillus flavus</i> Growth and Expression of Major Genes in Aflatoxin Biosynthetic Pathway | en |
| Type | Research Article | en |