Evaluation of False Negative Among SARS-COV-2 Patients with Negative Real-time PCR Result Using Nested-RT PCR
| Author | Mastaneh Alinezhadi | en |
| Author | Niloofar Neisi | en |
| Author | Mojtaba Rasti | en |
| Author | Maniya Arshadi | en |
| Author | Mehdi Parsanahad | en |
| Author | Bahman Cheraghian | en |
| Orcid | Niloofar Neisi [0000-0001-8224-4890] | en |
| Orcid | Mojtaba Rasti [0000-0002-9622-4839] | en |
| Orcid | Bahman Cheraghian [0000-0001-5446-6998] | en |
| Issued Date | 2022-06-30 | en |
| Abstract | Background: Fast and precise detection of SARS-CoV-2 RNA in clinical samples and subsequent quarantine are two critical factors in preventing virus transmission and distribution through the community. The false-negative result is a major problem in the SARS-CoV-2 detection because of the kind of sample (swab sample), sampling error, and sensitivity of PCR test, which can be reduced by a much more sensitive test such as nested PCR. Objectives: This study aimed to evaluate the false-negative rate among samples that were negative by a real-time PCR test using RT-nested PCR. Methods: One hundred eighty-four negative samples were included in the study, and nucleic acid was extracted using a commercial kit based on a silica filter column and then subjected to RT-nested PCR using three sets of primers targeting Orf1ab, N, and RdRp regions. Results: Among 184 negative swab samples for SARS-CoV-2, 27 (14.6%) cases were positive for the Orf1ab gene using RT-nested PCR. The samples were tested using N and RdRp primer sets. Also, seven (3.8%) cases were positive for the N gene, and four (2.1%) cases were positive for the RdRp gene. Conclusions: The results indicated that RT-nested PCR could be more sensitive than real-time PCR and reduce the false-negative rate. | en |
| DOI | https://doi.org/10.5812/jjm-122889 | en |
| Keyword | SARS-COV-2 | en |
| Keyword | COVID-19 | en |
| Keyword | Nested PCR | en |
| Keyword | False Negative | en |
| Publisher | Brieflands | en |
| Title | Evaluation of False Negative Among SARS-COV-2 Patients with Negative Real-time PCR Result Using Nested-RT PCR | en |
| Type | Research Article | en |