Detection of 13 Respiratory Pathogens via Capillary Electrophoresis-Based Multiplex PCR in Oropharyngeal Swabs and Nasal Swabs from Outpatients
| Author | Jiani Shan | en |
| Author | Tianlin Wang | en |
| Author | Xin Yang | en |
| Author | Qing Ye | en |
| Author | Yingshuo Wang | en |
| Author | Yifan Wang | en |
| Orcid | Tianlin Wang [0000-0002-6605-2204] | en |
| Orcid | Xin Yang [0000-0003-4267-9579] | en |
| Issued Date | 2025-02-28 | en |
| Abstract | Background: Respiratory tract infections are common in children, and capillary electrophoresis-based multiplex PCR (CEMP) is a promising method for detecting multiple pathogens simultaneously. However, its applicability to nasal swabs for detecting respiratory pathogens has not been thoroughly evaluated. Objectives: This study aimed to determine the distribution of 13 respiratory pathogens in outpatients with respiratory tract infections and to evaluate the clinical application value of nasal swab samples for pathogen detection using CEMP. Methods: A total of 153 outpatients with fever (temperature ≥ 38°C) accompanied by cough or sore throat were included in the study. Oropharyngeal swabs (OPSs) and nasal swabs were simultaneously collected from each patient. Capillary electrophoresis-based multiplex PCR was used to detect 13 respiratory pathogens, and the detection results of the two sample types were compared. Results: Of the 153 patients, 112 (73.2%) tested positive for at least one pathogen, with 83 (54.2%) having single infections and 29 (19.0%) having coinfections. The positive detection rates for nasal swabs and OPSs were 58.8% and 69.3%, respectively (P = 0.06). The total coincidence rate between the two sample types was 80.4%, with a Kappa value of 0.58 (P < 0.05). Conclusions: Capillary electrophoresis-based multiplex PCR effectively detects multiple pathogens causing respiratory tract infections in children. The results of nasal swabs and OPSs show good consistency, and nasal swab sampling is more comfortable and convenient, making it particularly suitable for children who may have difficulty cooperating during sample collection. | en |
| DOI | https://doi.org/10.5812/ijpediatr-159372 | en |
| Keyword | Oropharyngeal Swabs | en |
| Keyword | Nasal Swabs | en |
| Keyword | Respiratory Pathogen | en |
| Keyword | Capillary Electrophoresis-Based Multiple PCR | en |
| Keyword | Children | en |
| Keyword | Outpatients | en |
| Publisher | Brieflands | en |
| Title | Detection of 13 Respiratory Pathogens via Capillary Electrophoresis-Based Multiplex PCR in Oropharyngeal Swabs and Nasal Swabs from Outpatients | en |
| Type | Research Article | en |
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