Isohemagglutinin Titers and Clinical Significance in Blood Bank Donors: A Single-Center Cross-sectional Cohort Study

AuthorDidem Soydemiren
AuthorZeynep Karakaşen
AuthorMelek Yanaşık Nizamen
AuthorArzu Akcayen
AuthorGulyuz Ozturken
AuthorHikmet Gulsah Tanyildizen
OrcidZeynep Karakaş [0000-0002-8835-3235]en
OrcidMelek Yanaşık Nizam [0000-0003-1164-6774]en
OrcidArzu Akcay [0000-0003-0841-1667]en
OrcidGulyuz Ozturk [0000-0001-9268-8858]en
OrcidHikmet Gulsah Tanyildiz [0000-0002-0455-2078]en
Issued Date2025-10-31en
AbstractBackground: Isohemagglutinins directed against ABO blood group antigens play a crucial role in transfusion and transplantation. Understanding the distribution of isohemagglutinin titers in blood donors is critical, particularly in ABO-incompatible hematopoietic stem cell transplantation (HSCT). Objectives: The present study aimed to investigate the distribution of anti-A, anti-B, IgM, and IgG titers among healthy blood donors stratified by blood group, sex, and age. Methods: In this cross-sectional study, isohemagglutinin titers were measured in 1005 randomly selected donors (335 A, 335 B, and 335 O) at a single center. Titers were evaluated using the column agglutination technique, and statistical analyses were used to compare titer distributions by sex and age group. Results: The majority of donors were male (95.2%). Elevated anti-B IgM and IgG titers were significantly more common among female donors, particularly at 1:128 and 1:256 levels for IgM (P = 0.027; P = 0.012) and at the 1:1024 level for IgG (P = 0.005). The overall distribution of the ABO and Rh blood groups in this cohort was consistent with that reported in previous Turkish studies. Conclusions: Female donors demonstrated a tendency toward higher isohemagglutinin titers, although the small number of female participants limited definitive conclusions. These findings emphasize the need to screen high-titer donors, especially in the context of ABO-incompatible HSCT, and suggest that future longitudinal studies incorporating detailed immunologic histories are warranted.en
DOIhttps://doi.org/10.5812/ijpediatr-153406en
KeywordIsohemagglutinin Titeren
KeywordABO-Incompatible Transplantationen
KeywordBlood Donationen
KeywordHSCTen
KeywordSex Differencesen
PublisherBrieflandsen
TitleIsohemagglutinin Titers and Clinical Significance in Blood Bank Donors: A Single-Center Cross-sectional Cohort Studyen
TypeResearch Articleen

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