Evaluation of Leptin Receptor Q223R (rs1137101) Polymorphism Based on Histopathological and Body Mass Index Characteristics in Iranian Females with Breast Cancer
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Date
2020-04-30
0000-0002-9104-6308 Journal Title
Journal ISSN
Volume Title
Publisher
Brieflands
Abstract
Background: The mechanism of the relationship between obesity and the risk of breast cancer is still unknown. Objectives: The current study aimed at evaluating the relationship between the leptin receptor Q223R (Gln/Arg) polymorphism, body mass index (BMI), and histopathological changes in patients with breast cancer. Methods: A total of 158 patients with breast cancer and 158 healthy subjects within the same age range were recruited. After taking blood sample, DNAs were extracted using the salting-out method. Then, the polymerase chain reaction-restriction fragment length polymorphism technique was used for genotyping. The histopathological characteristics were determined by the immunohistochemistry technique. Results: Investigation of the relationship between BMI and alleles showed that the G (Arg) mutant elevated the risk of affection by 2.294 times, while the allele A had a descending effect by 0.375 times. The comparison of genotypes with histopathological changes showed a significant relationship between the GG (Arg/Arg) genotype and higher levels of progesterone receptor expression (P = 0.005), invasive type of cancer (P = 0.010), and stage of the disease (P = 0.003). Conclusions: People with higher BMI who also carried G (Arg) mutant of Q223R polymorphism were more prone to breast cancer. Also, tissue changes in the ones carrying this allele were more than those harboring the wild-type A (Gln) allele.