Simultaneous Detection of Multiple Respiratory Viruses Among SARS-CoV-2-Positive and Negative Patients by Multiplex TaqMan One-Step Real-Time PCR
| Author | Parsa Veisi | en |
| Author | Somayeh Shatizadeh Malekshahi | en |
| Author | Hamzeh Choobin | en |
| Author | Mohammad Reza Jabbari | en |
| Author | Peyman Mohammadi Torbati | en |
| Orcid | Somayeh Shatizadeh Malekshahi [0000-0003-2741-2745] | en |
| Issued Date | 2022-01-31 | en |
| Abstract | Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has clinical manifestations similar to other common respiratory viral infections. There are limited data on the frequency of viral respiratory coinfection among patients infected with SARS-CoV-2 in Iran. Objectives: This cross-sectional study investigated the prevalence of multiple respiratory viruses among SARS-CoV-2-positive and negative patients during the coronavirus disease 2019 (COVID-19) pandemic in Iran. Methods: We included oropharyngeal/nasopharyngeal swab specimens of patients suspected of COVID-19 from December 2020 to March 2021. A reliable multiplex TaqMan one-step real-time PCR method was employed to detect 17 viral respiratory pathogens simultaneously. Descriptive analyses were performed to characterize the specimens concerning age, gender, clinical manifestations, and underlying disease. Results: Multiple respiratory viruses with a frequency of 18.78% were detected in 197 studied patients. Human metapneumovirus was the most prevalent pathogen detected in both SARS-CoV-2-positive (n = 7, 7.7%) and negative (n = 7, 6.6%) patients. Moreover, the frequency rate of viral infection was almost the same in both SARS-CoV-2-positive (18.68%) and negative (18.86%) patients. Altogether, there were no differences in baseline demographic characteristics such as age, sex, clinical symptoms, and comorbidities between the two groups (P > 0.05). Conclusions: The data presented here expand our understanding of the epidemiology of multiple types of viral respiratory pathogens in suspected COVID-19 patients. Therefore, simultaneous screening of other viral respiratory pathogens will be helpful for clinicians and researchers interested in the control of viral respiratory tract infections. | en |
| DOI | https://doi.org/10.5812/jjm.122090 | en |
| Keyword | COVID-19 | en |
| Keyword | Coinfection | en |
| Keyword | Respiratory Infection | en |
| Keyword | SARS-CoV-2 | en |
| Keyword | Virology | en |
| Publisher | Brieflands | en |
| Title | Simultaneous Detection of Multiple Respiratory Viruses Among SARS-CoV-2-Positive and Negative Patients by Multiplex TaqMan One-Step Real-Time PCR | en |
| Type | Research Article | en |
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