Intracellular GSH Alterations and Its Relationship to Level of Resistance following Exposure to Cisplatin in Cancer Cells
| Author | Bardia Jamali | en |
| Author | Maryam Nakhjavani | en |
| Author | Leila Hosseinzadeh | en |
| Author | Salimeh Amidi | en |
| Author | Nastaran Nikounezhad | en |
| Author | Farshad H. Shirazi | en |
| Issued Date | 2015-04-30 | en |
| Abstract | One of the major complications in cancer chemotherapy with cisplatin as one of the important medicines in treatment regimens of different cancers is the development of resistance. One of the most described cellular defense mechanisms involved in resistance is glutathione (GSH), thus in this study, the effects of cisplatin on the total intracellular GSH level (GSHi) in some sensitive and resistant variants of human cell lines (hepatocarcinoma HepG2, skin A375, cisplatin sensitive glioblastoma U373MG and cisplatin resistant glioblastoma U373MGCP, cisplatin sensitive ovary A2780S and cisplatin resistant A2780CP cells) were studied. MTT assay was performed to measure cytotoxicity of cisplatin (33.3 µM for 1 hour). Following cisplatin exposure, GSHi (per million cells) was evaluated using a photometrical assay up to 90 minutes. Our results indicate that there are significant differences between GSHi content of A2780CP and U373MGCP cells compared to other cell lines. Moreover, IC50 of cisplatin in different cells seems to have a relation with mean of GSH level in 90 minutes (GSH (mean)90). | en |
| DOI | https://doi.org/10.22037/ijpr.2015.1661 | en |
| Keyword | A375 | en |
| Keyword | A2780s | en |
| Keyword | A2780CP | en |
| Keyword | HepG2 | en |
| Keyword | U373MGS | en |
| Keyword | U373 MGCP | en |
| Keyword | GSH | en |
| Keyword | Cisplatin | en |
| Publisher | Brieflands | en |
| Title | Intracellular GSH Alterations and Its Relationship to Level of Resistance following Exposure to Cisplatin in Cancer Cells | en |
| Type | Original Article | en |
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