Identification of a Novel Antibiotic from Myxobacterium <i>Stigmatella Eracta </i>WXNXJ-B and Evaluation of its Antitumor Effects <i>I</i><i>n</i><i>-</i><i>vitro</i>
| Author | Dahong Wang | en |
| Author | Jiangfeng Yuan | en |
| Author | Wenyi Tao | en |
| Issued Date | 2014-01-31 | en |
| Abstract | This work was to isolate and identify the bioactive secondary metabolite which was produced by myxobacterium Stigmatella eracta WXNXJ-B, and to evaluate its antitumor and apoptosis-inducing effects. The results showed that one novel compound (molecular formula C29H25NO3) was isolated, purified by Sephadex LH-20 column chromatography and preparative RP-HPLC, and identified as 5-(6-benzyl-quinolin-3-ylmethyl)-6- phenyl-3,7-dioxa- bicycle [4.1.0] heptan-3-one (named as quinoxalone) according to its UV, IR, HRMS and NMR spectra. The compound showed strong antitumor activity on B16, HepG2, MCF-7, SGC-7901, MDA-MB231 and CT-26 six tumor cell lines in-vitro. Nevertheless, it showed less cytotoxic to the mouse normal spleen cells (IC50 was 836.27 ± 13.02 µg mL-1). The cytotoxic study on HepG2 cells in-vitro showed that quinoxalone could induce the change of cell nuclear and arrested the cell division in the S and G2/M phase. Our results suggest that quinoxalone could be a potential anti-cancer agent. | en |
| DOI | https://doi.org/10.22037/ijpr.2014.1425 | en |
| URI | https://brieflands.com/journals/ijpr/articles/125425 | en |
| Keyword | Quinoxalone | en |
| Keyword | Myxobacterium<i> Stigmatella eracta</i> WXNXJ-B | en |
| Keyword | Structure identification | en |
| Keyword | Antitumor bioactivity | en |
| Publisher | Brieflands | en |
| Title | Identification of a Novel Antibiotic from Myxobacterium <i>Stigmatella Eracta </i>WXNXJ-B and Evaluation of its Antitumor Effects <i>I</i><i>n</i><i>-</i><i>vitro</i> | en |
| Type | Original Article | en |
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