Green-Synthesized Silver Nanoparticles Using Lavandula stricta Delile Exhibit Potent Anti-Inflammatory and Antioxidant Activities in Vivo
| Author | Kaveh Ebrahimi | en |
| Author | Mohammad Foad Noorbakhsh | en |
| Author | Mehdi Fazeli | en |
| Orcid | Kaveh Ebrahimi [0000-0002-1610-8297] | en |
| Orcid | Mohammad Foad Noorbakhsh [0000-0002-6983-0577] | en |
| Orcid | Mehdi Fazeli [0000-0003-0766-0514] | en |
| Issued Date | 2026-03-31 | en |
| Abstract | Background: The unique anti-inflammatory and antioxidant properties of silver nanoparticles (Ag-NPs) have made them a subject of growing scientific interest. Objectives: This study aimed to synthesize Ag-NPs using an aqueous extract of Lavandula stricta and comprehensively evaluate their anti-inflammatory and antioxidant activities in vivo. Methods: Ag-NPs were fabricated via a green synthesis route using L. stricta aqueous extract and 1 mM silver nitrate (AgNO3) as the precursor. The biosynthesized Ag-NPs were characterized by ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential analysis. In vitro antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging and ferric reducing antioxidant power (FRAP) assays, whereas in vivo anti-inflammatory efficacy was assessed using a carrageenan-induced murine paw edema model. Paw tissue homogenates were analyzed for tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), malondialdehyde (MDA), and total antioxidant capacity (TAC). Results: XRD confirmed the crystalline nature of the biosynthesized Ag-NPs, with crystallite sizes ranging from 13.8 to 18.7 nm, whereas TEM showed a mean particle diameter of 24 nm. UV-Vis spectroscopy revealed a characteristic surface plasmon resonance band centered at 400 nm. The biosynthesized nanoparticles exhibited substantial radical-scavenging activity (DPPH IC50 = 235 μg/mL); however, the crude extract showed superior in vitro antioxidant activity (IC50 = 120 μg/mL). In the carrageenan-induced paw edema model, Ag-NPs at 0.6 mg/kg produced 32.3% inhibition of paw swelling, accompanied by reductions in TNF-α (84.6%) and IL-6 (53%). Treatment with Ag-NPs also lowered MDA levels and increased TAC, with both oxidative stress markers reaching values comparable to those in the healthy control group (P > 0.05). Conclusions: These findings support the potential of L. stricta-mediated Ag-NPs as a candidate for managing acute inflammatory conditions and warrant further preclinical investigation. | en |
| DOI | https://doi.org/10.69107/jai-174480 | en |
| URI | https://brieflands.com/journals/jai/articles/174480 | en |
| Keyword | Carrageenan | en |
| Keyword | Paw Edema | en |
| Keyword | Tumor Necrosis Factor-alpha | en |
| Keyword | Interleukin-6 | en |
| Keyword | Malondialdehyde (MDA) | en |
| Keyword | Plant Extracts | en |
| Publisher | Brieflands | en |
| Title | Green-Synthesized Silver Nanoparticles Using <i>Lavandula stricta</i> Delile Exhibit Potent Anti-Inflammatory and Antioxidant Activities in Vivo | en |
| Type | Research Article | en |
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