Prevalence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. in Beef, Buffalo and Lamb Using Multiplex PCR
| Author | Mehdi Zarei | en |
| Author | Narjes Basiri | en |
| Author | Amirhesam Jamnejad | en |
| Author | Mohammad Hadi Eskandari | en |
| Orcid | Mehdi Zarei [0000-0002-2965-7773] | en |
| Issued Date | 2013-10-01 | en |
| Abstract | Background: Meat contamination has been linked to consumer health problems, as proved by outbreaks and recalls from market places . Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella spp. are considered among the most important pathogens which can be spread through meat and meat products consumption. Objectives: The aim of the present study was to determine the prevalence of E. coli O157:H7, L. monocytogenes, and Salmonella spp. in different kinds of meat marketed in Ahvaz, South-west part of Iran. Materials and Methods: A total of 210 samples of beef, buffalo and lamb meats were collected from retail outlets and popular supermarkets. After each single pathogen and DNA extraction, multiplex PCR as a rapid and cost-effective method was carried out to determine the prevalence of the pathogens in the samples. Results: L. monocytogenes was detected in 2.8% of beef and buffalo samples and 4.3% of lamb samples. E. coli O157:H7 was detected in 2.8% of beef and 1.4% of buffalo samples. However, no contamination with this pathogen was found in lamb samples. The prevalence of Salmonella spp. in beef, buffalo and lamb samples was 4.3, 2.8 and 7.1%, respectively. Conclusions: Due to the presence and potential hazard of E. coli O157:H7, L. monocytogenes, and Salmonella spp. in meat samples, the detection of these pathogens in different kinds of meat is crucial to safeguard public health. | en |
| DOI | https://doi.org/10.5812/jjm.7244 | en |
| Keyword | <i>Escherichia coli O157:H7</i> | en |
| Keyword | <i>Listeria monocytogenes</i> | en |
| Keyword | <i>Salmonella</i> | en |
| Keyword | Beef | en |
| Keyword | Buffalo | en |
| Keyword | Lamb | en |
| Publisher | Brieflands | en |
| Title | Prevalence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. in Beef, Buffalo and Lamb Using Multiplex PCR | en |
| Type | Research Article | en |
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