The rapid method of preparing mouse karyotype and mouse chromosomes identified by Gimsa staining and Trypsin-Gimsa banding
| Author | E Mirabzadeh Ardakani | en |
| Issued Date | 2001-03-31 | en |
| Abstract | Abstract Background: Preparation and staining of mouse chromosomes by Gimsa and T-G banding is the cornerstone of many researches on diagnosis, prevention and treatment of many diseases, production of new drugs and quality control of biological derivatives. Objective: To provide a sample karyotype of mouse. Methods: Mouse chromosomes were prepared from bone marrow of male outbred mice (NMRI). After culturing and preparing the slides, they were studied by staining with Gimsa and Trypsin-Gimsa (T-G) banding techniques. Karyotypes were prepared according to the standard karyotype of the mouse. Findings: Metaphase with spread from diploid cells contained 40 chromosomes and all of them were characterized by their banding patterns. Conclusion: T-G technique provides greather detail and demonstrates more bands, in contrast with other methods. | en |
| DOI | https://doi.org/ | en |
| Keyword | Keywords: Chromosome | en |
| Keyword | Karyotype | en |
| Keyword | Trypsin-Gimsa (T-G) banding | en |
| Keyword | Mouse | en |
| Publisher | Brieflands | en |
| Title | The rapid method of preparing mouse karyotype and mouse chromosomes identified by Gimsa staining and Trypsin-Gimsa banding | en |
| Type | Research Article | en |