A Survey of HBV Core/Pre-Core Mutations in Iraqi Patients with Chronic Hepatitis
| Author | Abdulhussain Kadhim Jwaziri | en |
| Author | Maryam Esghaei | en |
| Author | Mohammad Hadi Karbalaie Niya | en |
| Author | Hadi Sayah | en |
| Author | Mohammad Hossein Razizadeh | en |
| Author | Ali Gholami | en |
| Author | Leila Mousavizadeh | en |
| Author | Hossein Keyvani | en |
| Orcid | Maryam Esghaei [0000-0003-2944-2147] | en |
| Orcid | Mohammad Hadi Karbalaie Niya [0000-0002-1364-5743] | en |
| Orcid | Mohammad Hossein Razizadeh [0000-0003-3232-6163] | en |
| Orcid | Leila Mousavizadeh [0000-0002-7943-085X] | en |
| Orcid | Hossein Keyvani [0000-0001-8054-1939] | en |
| Issued Date | 2023-12-31 | en |
| Abstract | Objectives: The present study was conducted to assess the pattern of HBV Core/Pre-Core mutations and HBV genotype in Iraqi patients with chronic hepatitis B virus (HBV) infection. Methods: In the current cross-sectional study in an Iraqi province, we evaluated 134 patients diagnosed with HBV hepatitis. We used PCR and, subsequently, Sanger sequencing to assess HBV Core/Pre-Core mutations. Sanger sequencing reads were further used for phylogenetic analysis and multiple sequence alignment. A phylogenetic tree was generated according to the neighbor-joining method. Results: The current study revealed that 58 (45%) of the patients were male, and 72 (55%) of them were female. The mean age of the patients was 36 ± 12.7 years, and the mean duration of infection was 5.2 ± 4.8 years. The results revealed 21 nucleic acid alterations in the samples analyzed. The generated phylogenetic tree divided samples into two genotypes. Pre-core/Core mutations were significantly associated with the treatment received (P = 0.0.001) but not with laboratory parameters. Most samples were matched with the genotype D clade, while only four samples were positioned adjacent to the genotype E clade. Direct nucleic acid translation disclosed five nucleic acid variants (73T>G, 347T>G, 364A>G, 365T>C, and 366A>G) on the core protein. Conclusions: This study has detected 21 nucleotide variants and 5 amino acid alterations within the coding sequences of the C gene. This study revealed that genotype D represents the primary genotype for the identified viral infections. The current study highlights the importance of these mutations evaluation for future, more comprehensive studies. | en |
| DOI | https://doi.org/10.5812/hepatmon-137139 | en |
| Keyword | HBV | en |
| Keyword | Pre-Core/Core | en |
| Keyword | C Antigen | en |
| Keyword | Mutant | en |
| Keyword | Iraq | en |
| Publisher | Brieflands | en |
| Title | A Survey of HBV Core/Pre-Core Mutations in Iraqi Patients with Chronic Hepatitis | en |
| Type | Research Article | en |
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