Gene Expression and Pulmonary Toxicity of Chitosan-<i>graft</i>- Polyethylenimine as Aerosol Gene Carrier
| Author | Jung-Taek Kwon | en |
| Author | Hu-Lin Jiang | en |
| Author | Arash Minai-Tehrani | en |
| Author | Chang Gyu Woo | en |
| Author | Mansoo Choi | en |
| Author | Chong-Su Cho | en |
| Author | Yeon-Soo Kim | en |
| Author | Myung-Haing Cho | en |
| Issued Date | 2013-04-30 | en |
| Abstract | Chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer has been used for the improvement of low transfection efficiency of chitosan. The present study aims to test the pulmonary toxicity and efficiency of CHI-g-PEI as an aerosol gene carrier. Mice were exposed to aerosol containing green-fluorescent protein (GFP)-polyethylenimine (PEI) or GFP-CHI-g-PEI complexes for 30 min during the development of our nose-only exposure chamber (NOEC) system. CHI-g-PEI-mediated aerosol delivery demonstrated 15.65% enhancement of the fluorescence intensity. Compared to PEI, CHI-g-PEI showed no significant pulmonary toxicity. In summary, using CHI-g-PEI is safe and shows high transfection in aerosol gene delivery to animals, and enhanced efficiency was achieved through our aerosol gene delivery system. Therefore, CHI-g-PEI and this system would be applicable to future study for aerosol gene therapy. | en |
| DOI | https://doi.org/10.22037/ijpr.2013.1311 | en |
| Keyword | Aerosol delivery | en |
| Keyword | Chitosan | en |
| Keyword | Gene carrier | en |
| Keyword | Polyethylenimine | en |
| Keyword | Pulmonary toxicity | en |
| Publisher | Brieflands | en |
| Title | Gene Expression and Pulmonary Toxicity of Chitosan-<i>graft</i>- Polyethylenimine as Aerosol Gene Carrier | en |
| Type | Original Article | en |
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