A Recombinant Chimera Protein as a Novel <i>Brucella</i> Subunit Vaccine: Protective Efficacy and Induced Immune Response in BALB/c Mice
| Author | Abbas Abdollahi | en |
| Author | Shahla Mansouri | en |
| Author | Jafar Amani | en |
| Author | Mahdi Fasihi-Ramandi | en |
| Author | Reza Ranjbar | en |
| Author | Amir Ghasemi | en |
| Author | Mohammad Moradi | en |
| Orcid | Mahdi Fasihi-Ramandi [0000-0002-8242-4880] | en |
| Issued Date | 2018-01-31 | en |
| Abstract | Background: Brucellosis is one of the main causes of economic loss in domestic animals due to abortions and infertility. Brucella is a facultative intracellular pathogen that survives in other cell types in addition to phagocytes. T cell mediated responses are necessary to eradicate the infection due to Brucella. Objectives: In this study the potential of recombinant protein rTF/Bp26/Omp31 as a novel Brucella subunit vaccine, protective efficacy, and immune response was evaluated in BALB/c mice. Methods: After in silico design of rTF/Bp26/Omp31 structure, the gene was cloned and expressed in Escherichia coli BL21 (DE3). Finally, purified protein by Ni-NTA was used as immunogenic to immunized mice. Results: Mice immunized with rTF/Bp26/Omp31 showed a vigorous humoral and cellular mediated immunity; results were compatible with IgG1 and IgG2a levels as well as high amounts of IFN-γ, IL-12, IL-4 and IL-10 production in immunized mice, compared with control groups (P < 0.05). Compared to control groups, mice vaccinated with rTF/Bp26/Omp31 showed a significant response and protection against subsequent Brucella infection (P < 0.05). Conclusions: Statistical analyses indicate similar responses in immunized mice exposed to rTF/Bp26/Omp31, compared with B. abortus RB51 and B. melitensis Rev.1 vaccines. These results showed the potential of rTF/Bp26/Omp31 as a candidate for the development of a subunit vaccine against brucellosis. | en |
| DOI | https://doi.org/10.5812/jjm.12776 | en |
| Keyword | Vaccine | en |
| Keyword | Recombinant | en |
| Keyword | Omp31 Protein | en |
| Keyword | Trigger Factor (TF) | en |
| Keyword | Bp26 Protein | en |
| Keyword | <i>Brucella</i> | en |
| Publisher | Brieflands | en |
| Title | A Recombinant Chimera Protein as a Novel <i>Brucella</i> Subunit Vaccine: Protective Efficacy and Induced Immune Response in BALB/c Mice | en |
| Type | Research Article | en |