Cytotoxicity of ICD-85 NPs on Human Cervical Carcinoma HeLa Cells through Caspase-8 Mediated Pathway
| Author | Saeed Moradhaseli | en |
| Author | Abbas Zare Mirakabadi | en |
| Author | Ali Sarzaeem | en |
| Author | Morteza Kamalzadeh | en |
| Author | Reza Haji Hosseini | en |
| Issued Date | 2013-01-31 | en |
| Abstract | The biological application of nanoparticles (NPs) is a rapidly developing area of nanotechnology that raises new possibilities in the treatment of human cancers. The cytotoxicity was evaluated by MTT and LDH assays. The apoptotic effect of free ICD-85 and ICD-85 NPs on HeLa cells was assessed using caspase-8 colorimetric assay. The MTT assay showed that ICD-85 NPs could enhance the in-vitro cytotoxicity against HeLa cells compared to the free ICD-85. The IC50 value at 72 h was reduced from 25 ± 2.9 μg/mL for free ICD-85 to 15.5 ± 2.4 μg/mL for ICD-85 NPs. However, LDH assay demonstrated that ICD-85 has dose-dependent cytotoxicity on HeLa cells while ICD-85 NPs exhibited weaker cytotoxicity on same cells. The results also indicate that ICD-85-induced apoptosis on HeLa cells is associated with the activation of caspase-8. Moreover, caspase-8 assay analysis demonstrated that the ICD- 85 NPs induced a higher apoptotic rate in HeLa cells compared to free ICD-85. Our results demonstrated that the encapsulation of ICD-85 enhances its anti-proliferative effects. Taken together, these results suggest that the delivery of ICD-85 in nanoparticles may be a promising approach for the treatment of the cancer. | en |
| DOI | https://doi.org/10.22037/ijpr.2013.1253 | en |
| Keyword | Cancer | en |
| Keyword | Nanoparticles | en |
| Keyword | ICD-85 | en |
| Keyword | HeLa cell line | en |
| Keyword | MTT assay | en |
| Keyword | Caspase-8 | en |
| Publisher | Brieflands | en |
| Title | Cytotoxicity of ICD-85 NPs on Human Cervical Carcinoma HeLa Cells through Caspase-8 Mediated Pathway | en |
| Type | Original Article | en |