Effect of Ghrelin on Aldolase Gene Expression in the Heart of Chronic Hypoxic Rat
| Author | Mohammad Reza Aliparasti | en |
| Author | Mohammad Reza Alipour | en |
| Author | Shohreh Almasi | en |
| Author | Hadi Feizi | en |
| Issued Date | 2012-06-30 | en |
| Abstract | Background: Chronic hypoxia causes apoptosis of cardiac myocytes, however, energy production by anaerobic glycolysis protects myocardium against hypoxia injuries. Aldolase A is a well-characterised key enzyme of the glycolysis pathway. Ghrelin, a 28-amino-acid peptide, synthesizes in the stomach and has protective roles in cardiovascular systems and also affects metabolic pathways. Objectives: Therefore, the aim of this study was to evaluate the effect of ghrelin on aldolase A gene expression after chronic hypoxia in the rat hearts. Materials and Methods: Twenty four adult male wistar rats were randomly divided into three groups. Hypoxic rats with saline or ghrelin treatment were placed in a normobaric hypoxic chamber (O2 11 %), for two weeks. Controls remained in room air. Aldolase A gene expression was measured by Real-Time RT-PCR. Results: the transcriptiom rate of Aldolase A in hypoxic animals did not change significantly compared to negative control ones. During chronic hypoxia, ghrelin treatment increased the amount of heart Aldolase A gene expression compared to negative controls (P = 0.029). Hypoxic animals that were treated with ghrelin were significantly more polycythemic than the controls and even hypoxic with saline treated rats (P < 0.001). Conclusions: It seems that ghrelin interferes in the cardiac metabolism through up-regulation of glycolytic enzymes. In other words, it may protect heart from possible hypoxia induced damages. | en |
| DOI | https://doi.org/10.5812/ijem.3914 | en |
| Keyword | Ghrelin | en |
| Keyword | Cell Hypoxia | en |
| Keyword | Heart | en |
| Keyword | Glycolytic enzymes | en |
| Keyword | Rat | en |
| Publisher | Brieflands | en |
| Title | Effect of Ghrelin on Aldolase Gene Expression in the Heart of Chronic Hypoxic Rat | en |
| Type | Research Article | en |
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