Somatostatin Decorated Quantum Dots for Targeting of Somatostatin Receptors
| Author | Ahmed Abdelfattah Hafez Abdellatif | en |
| Author | Wael Abdellah Abdelhafez | en |
| Author | Hatem Abdelmunsef Sarhan | en |
| Issued Date | 2018-04-30 | en |
| Abstract | Due to the unique optical properties like high brightness and narrow emission bands of Quantum dots, it is used as simple fluorescence materials in bio-imaging, immunoassays, microarrays, and other applications. To easy invistigate cell lines that overexpressed somtostatin receptors, somatostatin (SST) was conjugated with Quantum dots carrying PEG amine (Qdots-PEG-NH2). The conjugation of SST to Qdots-PEG-NH2 started with the thiolation of SST using Traut’s reagent. Moreover, the Qdots-PEG-NH2 were subsequently activated by 500-fold molar excess of sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (sulfo-SMCC) dissolved in phosphate buffer. The Qdots-PEG-NH2-sulfo-SMCC was conjugated to the thiolated-SST to form Qdots-SST. The number of sulfhydryl groups can be controlled by the molar ratio of Traut´s reagent to SST. Thiolation was necessary for the conjugation of SST to Qdots-PEG-NH2. This was achieved by reacting the SST with Traut’s reagent in a 1:1 molar ratio. Ellman’s reagent was used to determine the number of sulfhydryle groups. Furthermore, cellular uptake study on triple negative breast cancer cells (HCC-1806) showed that the numbers of Qdots-SST per cell were significantly higher compared to unmodified Qdots-PEG-NH2 when quantified using inductively coupled plasma optical emission spectroscopy (ICP-OES). Moreover, the binding of Qdots-SST to cells can be suppressed by addition of free SST, indicating that the binding of Qdots-SST to cells is due to receptor-specific binding. | en |
| DOI | https://doi.org/10.22037/ijpr.2018.2189 | en |
| Keyword | Quantum dots | en |
| Keyword | Somatostatin receptors | en |
| Keyword | Somatostatin | en |
| Keyword | Cellular uptake | en |
| Keyword | Receptor targeting | en |
| Publisher | Brieflands | en |
| Title | Somatostatin Decorated Quantum Dots for Targeting of Somatostatin Receptors | en |
| Type | Original Article | en |
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