Apoptotic Effects of Ginger Extract (Zingiber officinale) on Esophageal Cancer Cells ESO26: An In Vitro Study
| Author | Abbas Abbasi | en |
| Author | Ali Azizi | en |
| Author | Seyed Mostafa Nachvak | en |
| Author | Elaheh Alizadeh | en |
| Author | Rezvan Abbsavaran | en |
| Author | Elham Mirtaheri | en |
| Author | Mehdi Moradinazar | en |
| Author | Mehrali Rahimi | en |
| Orcid | Ali Azizi [0000-0002-7128-4181] | en |
| Issued Date | 2020-12-31 | en |
| Abstract | Aim: Ginger is a natural dietary rhizome with antioxidant, anti-inflammatory, and anticarcinogenic properties. It has many medical beneficial properties such as anti-proliferation and antiapoptotic effects on cancerous esophageal cells. Materials and Methods: Esophageal cancer cells ESO26 were cultured in the presence and absence of ginger extract at various concentrations for 12, 18, and 24 h. Then, the viability was determined by 3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay. Western blot analysis of caspase-3 was performed to detect apoptosis. p21, Bax, and Bcl-2 gene expression was measured using quantitative polymerase chain reaction (PCR). Data were analyzed using one-way analysis of variance (ANOVA) followed by Tukey test. Results: The ginger extract increased the cleavage of caspase-3 in cells (P < 0.05). Results of real-time PCR have shown that ginger decreased the expression of Bcl-2 and increased Bax and p21 gene expression (P < 0.05). Conclusions: Results showed that the process of cell proliferation has been stopped. Also, this study indicated that ginger might exert a chemopreventive effect on esophageal cancer through the suppression of proliferation and the growth of tumor cells as well as the induction of apoptosis. | en |
| DOI | https://doi.org/10.4103/jrptps.JRPTPS_98_19 | en |
| URI | https://brieflands.com/journals/jrps/articles/147111 | en |
| Keyword | Apoptosis | en |
| Keyword | Caspase-3 | en |
| Keyword | esophageal cancer | en |
| Keyword | ginger | en |
| Publisher | Brieflands | en |
| Title | Apoptotic Effects of Ginger Extract (Zingiber officinale) on Esophageal Cancer Cells ESO26: An In Vitro Study | en |
| Type | Research Article | en |