MicroRNA Prediction in the FVIII Gene Locus: A Step Towards Hemophilia A Control
| Author | Halimeh Rezaei | en |
| Author | Majid Motovali-Bashi | en |
| Author | Sheyda Khalilian | en |
| Issued Date | 2020-07-27 | en |
| Abstract | Background: Various mutations in factor VIII (F8) gene locus are led to an X-linked bleeding disorder in patients with hemophilia A. One of the leading causes of inefficient treatment available for hemophilia A is the lack of specific and sensitive diagnostic procedure for the disease. The discovery of a functional role of microRNAs (miRNAs) in the pathogenesis of a wide range of human diseases makes them the potential, non-invasive, biomarker candidates for hemophilia A. Therefore, advances in computational tools for miRNA discovery leads to numerous recent publications on miRNAs as putative biomarkers. Objectives: The current study aimed at scanning the F8 gene region to predict novel miRNAs as regulators of the F8 gene. Methods: The potential of the FVIII locus to express new miRNAs was studied via reliable bioinformatics databases, such as SSCprofiler, RNAfold, miREval, miR-Find, FOMmiR, UCSC genome browser, and miRBase. Results: Data analysis from previously mentioned databases offered two stem-loop structures predicted to express novel miRNAs. Conclusions: The presented stem-loop structures can be used as powerful non-invasive biomarkers in early diagnosis of the disease and regulation of the factor VIII gene after subsequent experimental verification. | en |
| DOI | https://doi.org/10.5812/gct.103096 | en |
| URI | https://brieflands.com/journals/gct/articles/103096 | en |
| Keyword | microRNAs | en |
| Keyword | MiRNAs | en |
| Keyword | Bioinformatics, Hemophilia A | en |
| Keyword | Diagnosis | en |
| Keyword | Biomarker | en |
| Publisher | Brieflands | en |
| Title | MicroRNA Prediction in the FVIII Gene Locus: A Step Towards Hemophilia A Control | en |
| Type | Research Article | en |