Phylogenetic and 2D/3D Analysis of HCV 1a NS4A Gene/Protein in Pakistani Isolates
| Author | Abrar Hussain | en |
| Author | Muhammad Idrees | en |
| Author | Muhammad Asif | en |
| Author | Liaqat Ali | en |
| Author | Mahmood Rasool | en |
| Issued Date | 2015-06-01 | en |
| Abstract | Background: The nonstructural protein NS4A of hepatitis C virus is composed of 54 amino acids. This small size protein has vital role in many cellular functions. The most important reported function is being a cofactor of viral enzymes serine protease and helicase. Objectives: The objective of this study was to analyze the phylogenetic variation, its impact in terms of translation and any functional change in protein structure at primary 2D/3D structure using computational tools from Pakistani patients isolates. Material and Methods: Patient sera infected with Hepatitis C virus, genotype 1A, were obtained from Molecular Diagnostics lab, CEMB, University of the Punjab Lahore by using BD Vacutainer collection tubes (Becton Dickenson). Results: Phylogenetic analysis of the gene revealed that Pakistani 1a HCV strains are in the start of third cluster and there is a difference between inter Pakistani isolates at primary, secondary and tertiary levels. Conclusions: Mutations were present in the central domain of NS4A (amino acids 21 - 34). | en |
| DOI | https://doi.org/10.5812/hepatmon.15(6)2015.19936 | en |
| URI | https://brieflands.com/journals/hepatmon/articles/15420 | en |
| Keyword | Hepatitis C Virus | en |
| Keyword | Reverse Transcriptase Polymerase Chain Reaction | en |
| Keyword | RNA | en |
| Publisher | Brieflands | en |
| Title | Phylogenetic and 2D/3D Analysis of HCV 1a NS4A Gene/Protein in Pakistani Isolates | en |
| Type | Research Article | en |