Development and Use a Novel combined in-vivo and in-vitro Assay for Anti-inflammatory and Immunosuppressive Agents

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AuthorTan Lien
AuthorHong Chenen
AuthorXin Meien
AuthorNa Weien
AuthorBo Caoen
Issued Date2013-07-31en
AbstractContact hypersensitivity (CHS) mouse model induced by 2, 4-dinitrofluorobenzene (DNFB) is thought to be a T helper 1 (Th1)-dominant response and used for investigating anti-inflammatory and immunosuppressive agents. However, it is hardly used for screening large-scale drugs because of the large number of animals and complex mechanisms involved in-vivo. In this study, we focused on whether T lymphocytes from CHS mouse model could maintain the state of immune response in-vitro and explored a suitable time for drugs screening. The results showed that CD4+ T cells of CHS mice were higher compared with those in normal group. The expression of T-bet and GATA3 showed a Th1 shift and the levels of interleukin (IL)-2 and IL-4 also showed similar trend. Furthermore, IL-6 produced by T lymphocytes from CHS mice had a high level too. Then, we detected the effects of dexamethasone (DEX), cyclosporine A (CsA) and mycophenolate mofetil (MMF) on T lymphocytes in-vitro, and the data displayed that these immunosuppressive drugs could all inhibit the proliferation of T lymphocytes significantly. These findings suggested that T lymphocytes from CHS mice could mimic a similar immune response in-vitro, and it’s also a suitable method for screening anti-inflammatory and immunosuppressive agents.en
DOIhttps://doi.org/10.22037/ijpr.2013.1336en
URIhttps://brieflands.com/journals/ijpr/articles/125667en
KeywordImmunoregulationen
KeywordDelayed hypersensitivityen
KeywordT lymphocytesen
Keyword<i>in-vitro</i>en
PublisherBrieflandsen
TitleDevelopment and Use a Novel combined in-vivo and in-vitro Assay for Anti-inflammatory and Immunosuppressive Agentsen
TypeOriginal Articleen

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Iranian Journal of Pharmaceutical Research