Frequency and Molecular Detection of <i>Giardia intestinalis</i> in Children Attending Pediatrics of Punjab, Pakistan
| Author | Zeeshan Nawaz | en |
| Author | Muhammad Hidayat Rasool | en |
| Author | Abu Baker Siddique | en |
| Author | Muhammad Asif Zahoor | en |
| Author | Aneeqa Naz | en |
| Author | Sultan Ali | en |
| Author | Muhammad Uzair Mukhtar | en |
| Orcid | Zeeshan Nawaz [0000-0002-4673-2579] | en |
| Orcid | Abu Baker Siddique [0000-0002-8589-2292] | en |
| Orcid | Muhammad Asif Zahoor [0000-0002-0895-7312] | en |
| Orcid | Aneeqa Naz [0000-0002-1029-4765] | en |
| Orcid | Sultan Ali [0000-0001-8985-7729] | en |
| Orcid | Muhammad Uzair Mukhtar [0000-0003-0691-0518] | en |
| Issued Date | 2020-01-31 | en |
| Abstract | Background: Giardia intestinalis is the most common protozoan infecting the small intestine of human beings and a major cause of enteric infection, especially in children throughout the world. It is a highly diverse protozoan, which comprises a complex of eight genetic assemblages that are further differentiated into sub-assemblages. Objectives: A cross-sectional study was conducted to detect the frequency, molecular detection and assemblage identification of G. intestinalis in children of Punjab, Pakistan. Methods: A total of 800 stool samples were collected from children ranging 0 - 10 years of age with gastrointestinal disturbances and subjected to direct microscopy, enzyme-linked immunosorbent assay and polymerase chain reaction targeting small-subunit ribosomal RNA (SSU rRNA) and triosephosphate isomerase (tpi) genes. A predesigned questionnaire was filled prior to sampling from the guardian of each child to collect information. Results: The results indicated that the prevalence of 9.5% (76/800) was achieved by microscopy, ELISA and PCR targeting SSU-rRNA gene. The genetic DNA from 69 out of 76 (90.80%) was successfully amplified by tpi gene. Among these tpi gene-positive samples, 38 were successfully typed in assemblage B (55.07%) followed by 20 (28.98%) in assemblage A and 11 (15.94%) in mixed type assemblages (A & B). Residency and socioeconomic status were statistically associated with giardiasis. Among the clinical presentations, abdominal pain is prominent in assemblage B (57.89%) and vomiting in assemblage A (40%) type infections. Conclusions: Advanced molecular tools for giardiasis are well-adapted to get true prevalence, better discrimination of assemblages and their correlation with clinical signs. | en |
| DOI | https://doi.org/10.5812/jjm.97080 | en |
| Keyword | Molecular Detection | en |
| Keyword | <i>Giardia intestinalis</i> | en |
| Keyword | Assemblages | en |
| Keyword | Children | en |
| Keyword | Pakistan | en |
| Publisher | Brieflands | en |
| Title | Frequency and Molecular Detection of <i>Giardia intestinalis</i> in Children Attending Pediatrics of Punjab, Pakistan | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- jjm-13-1-97080.pdf
- Size:
- 126.12 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF