In-vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae)
| Author | Amir Sahraroo | en |
| Author | Mesbah Babalar | en |
| Author | Mohammad Hossein Mirjalili | en |
| Author | Mohammad Reza Fattahi Moghaddam | en |
| Author | Samad Nejad Ebrahimi | en |
| Issued Date | 2014-10-31 | en |
| Abstract | In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In-vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgL-1) solely or in combination with cytokinins BAP and KIN (1.0, 2.0 and 5.0 mgL-1). B5 medium supplemented with 1.0 mgL-1 IBA plus 5.0 mgL-1 BAP and MS medium fortified with 2.0 mgL-1 IBA and 2.0 mgL-1 BAP were the most favorable media for callus formation with the highest induction rate (96%). Maximum growth index (2.89 and 2.63) and maximum callus biomass (2.34 and 2.33 g fresh weight) were obtained from the callus cultured on B5 medium supplemented with 1.0 mgL-1 IBA plus 5.0 mgL-1 BAP and MS medium fortified with 1.0 mgL-1 IBA plus 1.0 mgL-1 KIN, respectively. Determination and quantification of RA in cultured calli were performed by HPLC UV/MS analysis. Calli induced from the plant and maintained on supplements of IBA and BAP in the absence of light produced RA 7.5% based on dry weight (DW). No differentiation was observed in any callus during the course of this study. | en |
| DOI | https://doi.org/10.22037/ijpr.2014.1570 | en |
| URI | https://brieflands.com/journals/ijpr/articles/125554 | en |
| Keyword | <i>Satureja</i> sp | en |
| Keyword | Lamiaceae | en |
| Keyword | Callus culture | en |
| Keyword | Rosmarinic acid | en |
| Keyword | HPLC UV/MS | en |
| Publisher | Brieflands | en |
| Title | <i>In-vitro</i> Callus Induction and Rosmarinic Acid Quantification in Callus Culture of <i>Satureja khuzistanica</i> Jamzad (Lamiaceae) | en |
| Type | Research Article | en |
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