Prevalence and Antimicrobial Susceptibility Patterns of ESBL, AmpC and Carbapenemase-producing <i>Enterobactericeae</i> Isolated from Hospitalized Patients in Azerbaijan, Iran
| Author | Reza Ghotaslou | en |
| Author | Mohammad Reza Sadeghi | en |
| Author | Mohammad Taghi Akhi | en |
| Author | Alka Hasani | en |
| Author | Mohammad Asgharzadeh | en |
| Issued Date | 2018-03-31 | en |
| Abstract | The current study was conducted to determine prevalence and antimicrobial susceptibility patterns, ESBL, AmpC and carbapenemase- producers among clinical isolates of Enterobacteriaceae. Three hundred and seven non-duplicative clinical isolates were collected from hospitalized patients in five medical centers in Azerbaijan, Iran. Initial screening for β-lactamase production was performed using disc agar diffusion according to Clinical Laboratory Standards Institute (CLSI) guidelines. Phenotypic confirmatory tests was done using total ESBL/AmpC confirm kit, KPC/MBL and OXA-48 confirm kit according to manufacturer’s instructions. The overall prevalence of ESBL, AmpC, and carbapenemase-producing strains were 42.7% (131/307), 14.0%(43/307) and 4.9% (15/307), respectively. The prevalence of ESBLs was 38.35%, 64.9% and 35.7% for E. coli, K. pneumoniae and E. cloacae, respectively. Carbapenemase activity was only observed among 15 K. pneumoniae isolates and detected phenotypes include MBL (9/57, 15.8%), KPC (4/57, 7.0%), and OXA-48 (2/57, 3.5%). Fourteen out of 307 isolates (4.6%) were recognized to have ESBL or AmpC hyper-producer with decreased cell wall permeability phenotype. All 4 shigella strains were positive for ESBL and 4 isolates of P. mirabilis, 2 isolates of M. morganii and 1 seratia spp. were detected as AmpC producer. The only C. freundii strain isolated was positive for both ESBL and AmpC. This study reveals high prevalence of multidrug-resistant (MDR) β-lactamase-producing Enterobactericeae reaching 89.5%. Imipenem and meropenem showed potent antibacterial activities against all MDR β-lactamase-producers except for carbapenemase producers. After carbapenems, amikacin, piperacillin/tazobactam and amoxicillin/clavulanic acid were second the most effective drugs against β-lactamase-producing E. coli isolates. | en |
| DOI | https://doi.org/10.22037/ijpr.2018.2206 | en |
| Keyword | Antibiotic resistance | en |
| Keyword | β-lactamase-producing <i>Enterobactericeae</i> | en |
| Keyword | Hospitalized patients | en |
| Keyword | Azerbaijan | en |
| Keyword | Iran | en |
| Publisher | Brieflands | en |
| Title | Prevalence and Antimicrobial Susceptibility Patterns of ESBL, AmpC and Carbapenemase-producing <i>Enterobactericeae</i> Isolated from Hospitalized Patients in Azerbaijan, Iran | en |
| Type | Original Article | en |
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