<i>Clostridium perfringens</i> Spores in Urology Hospital
| Author | Zygmunt Gofron | en |
| Author | Klaudia Szarek | en |
| Author | Małgorzata Aptekorz | en |
| Author | Monika Kabała | en |
| Author | Gayane Martirosian | en |
| Orcid | Zygmunt Gofron [0000-0002-3601-1356] | en |
| Orcid | Klaudia Szarek [0000-0002-2202-5466] | en |
| Orcid | Małgorzata Aptekorz [0000-0002-7343-4702] | en |
| Orcid | Monika Kabała [0000-0003-4234-586X] | en |
| Orcid | Gayane Martirosian [0000-0002-0311-0884] | en |
| Issued Date | 2022-05-31 | en |
| Abstract | Background: Clostridium spp. spores are resistant to many factors, including alcohol-based disinfectants. The presence of clostridial spores in a hospital environment may lead to infection outbreaks among patients and health care workers. Objective: This study is aimed to detect clostridial spores in the aurology hospital using C diff Banana Broth™ and assess the antibiotic sensitivity and toxinotypes of isolates. Methods: After diagnosing COVID-19 in medical staff and closing an 86-bed urology hospital in 2020 for H2O2 fogging, 58 swabs from the hospital environment were inoculated to C diff Banana Broth™, incubated at 37°C for 14 days, checked daily, and positive broths were sub-cultured anaerobically for 48 h at 37°C. After identification, multiplex PCR (mPCR) was performed for Clostridium perfringens, C. difficile toxin genes, and minimum inhibitory concentration (MIC) determination. Results: In this study, 16 out of 58 (~ 28%) strains of Clostridium spp. were cultured: 11 - C. perfringens, 2 - C. baratii, and 1 each of C. paraputrificum, C. difficile, and C. clostridioforme. 11 C. perfringens were positive for the cpa, 7 - the cpb2, 2 – cpiA, and 1 – cpb toxin genes. All isolates were sensitive to metronidazole, vancomycin, moxifloxacin, penicillin/tazobactam, and rifampicin. Two out of the 11 C. perfringens strains were resistant to erythromycin and clindamycin. Conclusions: Regardless of the performed H2O2 fogging, antibiotic-resistant, toxigenic strains of C. perfringens (69%) obtained from the urology hospital environment were cultured using C diff Banana Broth™, indicating the need to develop the necessary sanitary and epidemiological procedures in this hospital. | en |
| DOI | https://doi.org/10.5812/jjm-124129 | en |
| Keyword | <i>Clostridium perfringens</i> | en |
| Keyword | <i>Clostridium difficile</i> | en |
| Keyword | Spores of Hospital Environment | en |
| Keyword | C diff Banana Broth | en |
| Publisher | Brieflands | en |
| Title | <i>Clostridium perfringens</i> Spores in Urology Hospital | en |
| Type | Research Article | en |
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