Genotyping of Hepatitis B Virus by Multiplex PCR in Sistan and Baluchestan Province
| Author | Kajal Yoosefi | en |
| Author | Mohsen Taheri | en |
| Author | Soheila Khosravi | en |
| Author | Esmaeel Saneie-Moghadam | en |
| Author | Amir Hossein Mohagheghi-Fard | en |
| Orcid | Mohsen Taheri [0000-0002-1110-4417] | en |
| Orcid | Amir Hossein Mohagheghi-Fard [0000-0001-5785-4419] | en |
| Issued Date | 2016-02-29 | en |
| Abstract | Background: Hepatitis B virus (HBV) has been classified into 8 genotypes (A - H) according to at least 8% difference in complete nucleotide sequences or more than 4% in S gene. Different genotypes of HBV have important roles in development of certain disorders as: hepatitis, cirrhosis and hepatocellular carcinoma. Therefore, knowing the distribution of HBV genotypes, have considerable impacts on treatment strategies, vaccination program, diagnosis and prevention. Objectives: This study aims to determine the prevalence of HBV genotypes in patients infected with the virus in Sistan and Baluchestan province (south-east Iran). Patients and Methods: In this descriptive study, 163 patients infected with HBV were selected over the first 6 months (beginning from March 2012 onward). All the patients were HBsAg (hepatitis B surface antigen) and HBeAg (extracellular form of HBcAg) positive along with viral load > 10000 copies/mL. After DNA extraction from the patients’ sera, viral genotypes were determined by multiplex polymerase chain reaction (PCR) using special primers for each genotype. Results: From 163 samples, 154 samples (94.5%) had D genotype which was dominant genotype; and 9 samples (5.5%) had mixed genotype of C/D. Conclusions: This study showed that in Sistan and Baluchestan, like other regions of Iran as well as Mediterranean countries, D genotype is dominant genotype. In additional, C/D mixed genotype is also seen less frequently in this province. | en |
| DOI | https://doi.org/10.17795/zjrms-5986 | en |
| Keyword | Genotype | en |
| Keyword | Hepatitis B Virus | en |
| Keyword | Multiplex | en |
| Keyword | Polymerase Chain Reaction | en |
| Publisher | Brieflands | en |
| Title | Genotyping of Hepatitis B Virus by Multiplex PCR in Sistan and Baluchestan Province | en |
| Type | Research Article | en |
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