[Retracted Article] Methylation of the Wnt Signaling Antagonist, Wnt Inhibitory Factor 1 and Dickkopf-1 Genes in Acute Myeloid Leukemia at the Time of Diagnosis
| Author | Ali Ghasemi | en |
| Author | Abbas Ghotaslou | en |
| Author | Mohsen Mohammadi | en |
| Author | Sadegh Abbasian | en |
| Author | Kazem Ghaffari | en |
| Orcid | Ali Ghasemi [0000-0002-4996-7656] | en |
| Issued Date | 2016-01-31 | en |
| Abstract | Background: In acute myeloid leukemia (AML), a large number of tumor suppressor genes are silenced through DNA methylation such as CDKN2B and p73. Wnt inhibitory factor 1 (WIF1) and Dickkopf-1 (DKK-1) are negative regulator of the Wnt signaling pathway. Objectives: In the present study, we studied the methylation status of WIF1 and DKK-1 genes in AML patients. Patients and Methods: In this case-control study, blood samples from 120 AML patients and 25 healthy control subjects collected, isolated DNA was treated with sodium bisulphite and examined by methylation specific PCR (MS-PCR) with primers specific for methylated and unmethylated sequences of the WIF1 and DKK-1 genes. Results: The frequency of aberrant hypermethylation of WIF1 and DKK-1 genes in AML patients determined 35% (42/120) and 28.3% (34/120), respectively. In addition, for all subjects in control group, methylation of WIF1 and DKK-1 genes were negative. Patients with M0 subtype of French-American-British (FAB)-AML had the highest incidence of hypermethylation of WIF1 (P = 0.003) and DKK-1 (P = 0.005) genes. Conclusions: The present study showed that, like many solid tumors, WIF1 and DKK-1 genes methylation also occurs in AML. The study of other antagonists of Wnt signaling pathway are recommended. | en |
| DOI | https://doi.org/10.17795/zjrms-5874 | en |
| Keyword | Acute Myeloid Leukemia | en |
| Keyword | Wnt Inhibitory Factor 1 | en |
| Keyword | Dickkopf-1 | en |
| Keyword | DNA Methylation | en |
| Publisher | Brieflands | en |
| Title | [Retracted Article] Methylation of the Wnt Signaling Antagonist, Wnt Inhibitory Factor 1 and Dickkopf-1 Genes in Acute Myeloid Leukemia at the Time of Diagnosis | en |
| Type | Research Article | en |
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