In-vitro Assessment of the Antiproliferative and Apoptotic Potential of the Ethyl acetate Extract of Peltophorumafricanum on Different Cancer Cell Lines
| Author | Benjamin Ifeoluwa Okeleye | en |
| Author | Noxolo Thabiso Mkwetshana | en |
| Author | Roland Ndip Ndip | en |
| Issued Date | 2017-04-30 | en |
| Abstract | We evaluated the in-vitro antiproliferative and apoptotic potential of the ethyl acetate extract (EAE) of Peltophorum africanum, a member of the family Fabaceae (Sond) in order to validate its pharmacological use. Antiproliferation of human breast (MCF-7), colon (HT-29) and cervical (HeLa) cancer cell lines by EAE was investigated using the Cell Titer-Blue viability assay and the mechanism of action delineated using the Nucleic Acid and Protein Purification Nucleospin® Tissue Kit, Scanning Electron Microscope (SEM), propidium iodide (PI) and acridine orange (AO) double-staining techniques. We observed a significant reduction in cell viability of the MCF-7 cells from 100% (untreated) to 54.33 ± 1.84% after 72 h of treatment with 5 µg/mL of EAE (P. value < 0.05). Internucleosomal DNA of MCF-7, HT-29 and HeLa cells was randomly fragmented into an uninterrupted spectrum of sizes, complemented by the intercalation of nucleic acid-specific fluorochromes by PI and AO spotting two phases of apoptosis; early (EA) and late (LA) apoptosis. Distinctive ultramorphological changes observed included; cell shrinkage, membrane blebbing, and typical cell induced death. The ethyl acetate extract of P.africanum has the potential to induce apoptosis and is undergoing further studies in-vivo as a likely template for new anticancer therapy. | en |
| DOI | https://doi.org/10.22037/ijpr.2017.1833 | en |
| URI | https://brieflands.com/journals/ijpr/articles/124996 | en |
| Keyword | <i>Peltophorum africanum</i> | en |
| Keyword | Medicinal plant | en |
| Keyword | Antiproliferation | en |
| Keyword | apoptosis | en |
| Keyword | Chemotherapy | en |
| Publisher | Brieflands | en |
| Title | <i>In</i><i>-</i><i>vitro</i> Assessment of the Antiproliferative and Apoptotic Potential of the Ethyl acetate Extract of <i>Peltophorum</i><i>africanum</i> on Different Cancer Cell Lines | en |
| Type | Research Article | en |
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