A Simple and Specific Stability- Indicating RP-HPLC Method for Routine Assay of Adefovir Dipivoxil in Bulk and Tablet Dosage Form
| Author | Bahar Darsazan | en |
| Author | Alireza Shafaati | en |
| Author | Seyed Alireza Mortazavi | en |
| Author | Afshin Zarghi | en |
| Issued Date | 2017-01-31 | en |
| Abstract | A simple and reliable stability-indicating RP-HPLC method was developed and validated for analysis of adefovir dipivoxil (ADV).The chromatographic separation was performed on a C18 column using a mixture of acetonitrile-citrate buffer (10 mM at pH 5.2) 36:64 (%v/v) as mobile phase, at a flow rate of 1.5 mL/min. Detection was carried out at 260 nm and a sharp peak was obtained for ADV at a retention time of 5.8 ± 0.01 min. No interferences were observed from its stress degradation products. The method was validated according to the international guidelines. Linear regression analysis of data for the calibration plot showed a linear relationship between peak area and concentration over the range of 0.5–16 μg/mL; the regression coefficient was 0.9999and the linear regression equation was y = 24844x–2941.3. The detection (LOD) and quantification (LOQ) limits were 0.12 and 0.35 μg/mL, respectively. The results proved the method was fast (analysis time less than 7 min), precise, reproducible, and accurate for analysis of ADV over a wide range of concentration. The proposed specific method was used for routine quantification of ADV in pharmaceutical bulk and a tablet dosage form. | en |
| DOI | https://doi.org/10.22037/ijpr.2017.2006 | en |
| Keyword | Adefovir Dipivoxil | en |
| Keyword | Assay | en |
| Keyword | RP-HPLC | en |
| Keyword | Stability-Indicating | en |
| Keyword | Pharmaceutical dosage form | en |
| Publisher | Brieflands | en |
| Title | A Simple and Specific Stability- Indicating RP-HPLC Method for Routine Assay of Adefovir Dipivoxil in Bulk and Tablet Dosage Form | en |
| Type | Original Article | en |
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