Evaluation of Recombinant SRS3 Antigen for Diagnosis of Toxoplasmosis by Enzyme-Linked Immunosorbent Assay
| Author | Seyedeh Mahya Marashiyan | en |
| Author | Fatemeh Moradian | en |
| Author | Geita Saadatnia | en |
| Author | Majid Golkar | en |
| Issued Date | 2017-01-31 | en |
| Abstract | Background: Infections by the protozoan parasite Toxoplasma gondii (T. gondii) are widely prevalent in human and animals. SRS3 is a member of SRS antigen family (glycosylphosphatidylinositol-linked proteins), which is structurally related to the highly immunogenic surface antigen SAG1. The SAG family of proteins can cover the surface of T. gondii bradyzoites and tachyzoites. Objectives: The aim of this study was to evaluate the recombinant SRS3 protein for diagnosis of toxoplasmosis by enzyme-linked immunosorbent assay. Methods: Indirect immunoglobulin G (IgG) enzyme-linked immunosorbent assays (ELISAs) with recombinant SRS3 and SAG1 proteins of T. gondii were developed to evaluate the diagnosis of infection in pregnant women. Results: The results showed that sensitivity and specificity were respectively 84.12% and 92% for recombinant protein SRS3 and 92% and 96% for rSAG1. Conclusions: Recombinant proteins SRS3 and rSAG1 are promising antigens in diagnostic assays for detection of specific antibodies against T. gondii. | en |
| DOI | https://doi.org/10.5812/archcid.35612 | en |
| Keyword | <i>Toxoplasma gondii</i> | en |
| Keyword | Recombinant Protein SRS3 | en |
| Keyword | Recombinant Protein SAG1 | en |
| Keyword | ELISA | en |
| Publisher | Brieflands | en |
| Title | Evaluation of Recombinant SRS3 Antigen for Diagnosis of Toxoplasmosis by Enzyme-Linked Immunosorbent Assay | en |
| Type | Research Article | en |
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