Characteristics and Hepatoprotective Activity of the Curcuma heyneana Rhizome Extract toward Wistar Rats Induced by Ethanol
| Author | Marianne Marianne | en |
| Author | Mariadi Mariadi | en |
| Author | Sony Eka Nugraha | en |
| Author | Rosnani Nasution | en |
| Author | Pran Nando Syuhada | en |
| Author | Sylvia Pandiangan | en |
| Orcid | Marianne Marianne [0000-0003-4378-8611] | en |
| Issued Date | 2021-11-30 | en |
| Abstract | Background: Curcuma heyneana (Valeton & Zijp.) or temu giring has various pharmacological activities. However, its hepatoprotective activity toward ethanol induction has never been carried out. Objectives: The objective of this research was to evaluate the hepatoprotective activity of the C. heyneana rhizome extract toward Wistar rats induced by ethanol. Methods: The research was initiated with the determination of curcuminoid content, total phenolic content, total flavonoid content, and characterization of extract using gas chromatography-mass spectrometry (GC-MS). Hepatoprotective activity was tested using the C. heyneana extract at doses of 50, 100, and 150 mg/kg with 5 g/kg ethanol as an inducer. Aspartate transaminase (AST), alanine transaminase (ALT), liver weight, and macroscopic and microscopic liver were used as parameters. Data were analyzed using analysis of variance (ANOVA). Results: The curcuminoid content of the extract was 1.18% (w/w). Total phenolic content of the C. heyneana extract was 400.37 mg gallic acid equivalent (GAE)/g sample, while total flavonoid content was 27.25 mg quercetin equivalent (QE)/g sample. Nine compounds were identified in the extract. Administration of the extract at doses of 50, 100, and 150 mg/kg kept the liver normal. It was identified macroscopically from the dark red color without any white spot and normal liver weight. Furthermore, at doses of 50, 100, and 150 mg/kg, the extract inhibited AST and ALT elevation, which was significantly different from the negative control group (P < 0.05). The extract also prevented hepatocyte injury that was seen microscopically. Conclusions: It can be concluded that the C. heyneana extract at doses of 50, 100, and 150 mg/kg is effective as hepatoprotective in the liver injury induced by ethanol. | en |
| DOI | https://doi.org/10.5812/jjnpp.112653 | en |
| URI | https://brieflands.com/journals/jjnpp/articles/112653 | en |
| Keyword | Hepatoprotective | en |
| Keyword | <i>Curcuma heyneana</i> | en |
| Keyword | Total Phenolic Content | en |
| Keyword | Total Flavonoid Content | en |
| Keyword | Liver Injury | en |
| Publisher | Brieflands | en |
| Title | Characteristics and Hepatoprotective Activity of the <i>Curcuma heyneana</i> Rhizome Extract toward Wistar Rats Induced by Ethanol | en |
| Type | Research Article | en |