Study of the Central Injection Effects of Chrysin on Behavioral and Intra Hypothalamic Gene Expression Levels of <i>CRH</i> and <i>CGRP</i> in Male Rats
| Author | Khadijeh Haghighat | en |
| Author | Fariba Mahmoudi | en |
| Author | Homayoun Khazali | en |
| Orcid | Khadijeh Haghighat [0000-0002-9016-3086] | en |
| Orcid | Fariba Mahmoudi [0000-0001-6092-1352] | en |
| Orcid | Homayoun Khazali [0000-0002-3231-0463] | en |
| Issued Date | 2024-04-30 | en |
| Abstract | Background: Chrysin is a natural flavonoid with several demonstrated neuro-pharmacological effects in brain areas related to anxiety. However, the intra-hypothalamic molecular mechanisms underlying the anxiolytic effects of chrysin remain unclear. Objectives: The current study revealed the effects of chrysin on hypothalamic corticotrophin-releasing hormone (CRH) and calcitonin gene-related peptide (CGRP) gene expression levels in a rat model of stress. Methods: Thirty male Wistar rats weighing 200 ± 10 g were divided into six groups for this investigation. Acute restraint stress was induced in the animals for 2 hours. Intact or stress-induced rats received 20 or 40 µg of chrysin via the third cerebral ventricle, respectively. Open-field and forced swimming tests were performed to evaluate stress-related behaviors. Hypothalamic samples were then removed, and real-time polymerase chain reaction (PCR) was used to measure relative gene expression. Results: The results showed that in the rats receiving chrysin, CRH and CGRP gene expression levels were significantly decreased compared to the stress group. Additionally, chrysin injection reduced anxiogenic behaviors. Conclusions: Chrysin decreased the expression of hypothalamic CRH and CGRP genes in stressed rats. | en |
| DOI | https://doi.org/10.5812/gct-147106 | en |
| Keyword | <i>CGRP</i> | en |
| Keyword | Chrysin | en |
| Keyword | <i>CRH</i> | en |
| Keyword | Stress | en |
| Publisher | Brieflands | en |
| Title | Study of the Central Injection Effects of Chrysin on Behavioral and Intra Hypothalamic Gene Expression Levels of <i>CRH</i> and <i>CGRP</i> in Male Rats | en |
| Type | Research Article | en |
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