Cloning and Expression of Brucella Cyclic ?-1, 2 Glucan Transporter Gene (cgt)
| Author | Mojgan Bandehpour | en |
| Author | Narges Abdali | en |
| Author | Farzaneh Sadeghi | en |
| Author | Kazem Parivar | en |
| Author | Zarrin Sharifnia | en |
| Author | Hossein Ryahi | en |
| Author | Ali Haghighi | en |
| Author | Bahram Kazemi | en |
| Orcid | Bahram Kazemi [0000-0002-3072-8831] | en |
| Issued Date | 2009-01-31 | en |
| Abstract | Background: Brucellosis is an important cosmopolitan infection disease caused by organisms belonging to the genus Brucella. The cgt gene (cyclic ?-1, 2 glucan transporter gene) is a virulent factor in Brucella genus. The present study was conducted with the aim of cloning and expression of Brucella cgt gene. Materials and methods: Brucella melitensis cgt gene was amplified from extracted chromosomal DNA by PCR, then : PCR product was cloned into pTZ57R and subcloned into pGEMEX-1 expression vector, then expressed in JM109 E.coli strain. Recombinant protein was confirmed by western blot analysis using patient's serum. Results: The PCR product was cloned in pTZ57R plasmid via T/A cloning method. Recombinant plasmid was digested by BamHI and SacI restriction enzymes, the released band was purified and subcloned into pGEMEX-1 expression vector. Then, sample cells were lysed using lyses buffer and sonicated, then electrophoresed on SDS-PAGE. Protein bands were transferred on nitrocellulose membrane and reacted by patient's serum and detected by HRP conjugated anti : human antibody. Conclusion: We cloned and expressed Brucella abortus cyclic -1, 2-glucan transporter gene (cgt) which is an important agent in brucellosis. Using cgt gene mutant may be an effective way for inhibiting or decreasing the pathogenicity of bacteria | en |
| DOI | https://doi.org/ | en |
| Keyword | Brucella | en |
| Keyword | Cyclic beta 1-2 glucan transporter | en |
| Keyword | Recombinant protein. | en |
| Publisher | Brieflands | en |
| Title | Cloning and Expression of Brucella Cyclic ?-1, 2 Glucan Transporter Gene (cgt) | en |
| Type | Research Article | en |
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