Vitamin D Inhibition of TRPV5 Expression During Osteoclast Differentiation
| Author | Jianhong Gu | en |
| Author | Xishuai Tong | en |
| Author | Yang Chen | en |
| Author | Chuang Zhang | en |
| Author | Tianhong Ma | en |
| Author | Saihui Li | en |
| Author | Wenyan Min | en |
| Author | Yan Yuan | en |
| Author | Xuezhong Liu | en |
| Author | Jianchun Bian | en |
| Author | Zongping Liu | en |
| Issued Date | 2019-10-31 | en |
| Abstract | Background: Vitamin D is an important steroid that can regulate bone metabolism including osteoclast (OC) differentiation. Transient receptor potential cation channel subfamily V member 5 (TRPV5), is a calcium channel protein involved in OC differentiation. However, the impact of vitamin D on TRPV5 expression during OC differentiation is not clear. Objectives: To determine if 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) regulates the expression of TRPV5 during OC differentiation. Methods: Bone marrow mononuclear macrophage (BMMs) were induced to differentiate into OC with or without treatment with 10 nM 1,25(OH)2D3. The expression levels of vitamin D receptor (VDR) and TRPV5 were examined. The expression of several OC markers, including tartrate resistant acid phosphatase (TRAP), carbonic anhydrase II (Ca II), cathepsin K (CTSK), and vacuolar-type H+-ATPase (V-ATPase) were also detected. Results: We found that the VDR was expressed in murine bone marrow-derived macrophages at the early stage of OC differentiation. TRPV5 expression was increased during OC differentiation, which was down-regulated by 1,25(OH)2D3 after a prolonged exposure. The 1,25(OH)2D3 and TRPV5 inhibitors inhibited OC differentiation. Conclusions: 1,25(OH)2D3 can inhibit TRPV5 expression as well as TRPV5 inhibitors during OC differentiation. This suggests that 1,25(OH)2D3 may suppress OC differentiation by inhibiting TRPV5 expression. | en |
| DOI | https://doi.org/10.5812/ijem.91583 | en |
| Keyword | Osteoclast | en |
| Keyword | 1 | en |
| Keyword | 25(OH)<sub>2</sub>D<sub>3</sub> | en |
| Keyword | TRPV5 | en |
| Keyword | RANKL | en |
| Keyword | Ca<sup>2+</sup> | en |
| Publisher | Brieflands | en |
| Title | Vitamin D Inhibition of TRPV5 Expression During Osteoclast Differentiation | en |
| Type | Research Article | en |