The Combined Effect of High-intensity Interval Training and Soy Isoflavone Diet on MicroRNA-133 Gene in Ovariectomized Rats
| Author | Lamia Mirheidari | en |
| Author | Rahman Soori | en |
| Author | Ali Asghar Ravasi | en |
| Author | Morteza Jourkesh | en |
| Orcid | Ali Asghar Ravasi [0000-0002-5549-2132] | en |
| Issued Date | 2022-09-30 | en |
| Abstract | Background: The combination of exercise and soy extract supplementation decreases cardiac related disease risk in postmenopausal women. Objectives: We assessed the combined impacts of soy isoflavone (IF) supplementation and exercise on the expression of microRNA-133 gene in ovariectomized rats’ hearts. Methods: This study was conducted on 50 female Wistar rats, which were assigned to five groups, including sham-operated (SHAM), ovariectomized (OVX), ovariectomized with soy IF supplementation (OVX + IF), OVX with exercise (OVX + HIIT), and OVX with both soy IFs and exercise (OVX + IF + HIIT). The experimental rats underwent high-intensity interval training (5 days/week) and/or supplied a soy IF supplementation for six weeks. The cardiac microRNA-133 gene expression was analyzed using real-time PCR after six weeks of intervention. Results: Ovariectomy down-regulated cardiac microRNA133 gene expression, but 42 days of IF administration or HIIT reversed this effect (P > 0.05). The HIIT reversed estrogen-deficiency-related microRNA-133 down-regulation greater than IF or combining IF and HIIT (P > 0.05). The OVX + IF and OVX + IF + HIIT (P > 0.05) showed no significant difference. Conclusions: Our results suggested that treatment with exercise, soy IF, and the combined treatment could benefit microRNA-133 in ovariectomized rats. This effect was significant when exercise alone was used to induce treatment. Thus, these treatments could offer protection in the menopausal period. | en |
| DOI | https://doi.org/10.5812/jkums-122938 | en |
| Keyword | Exercise Training | en |
| Keyword | Isoflavone | en |
| Keyword | Menopause | en |
| Keyword | Ovariectomy | en |
| Keyword | Rat Model | en |
| Publisher | Brieflands | en |
| Title | The Combined Effect of High-intensity Interval Training and Soy Isoflavone Diet on MicroRNA-133 Gene in Ovariectomized Rats | en |
| Type | Research Article | en |
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