Melatonin Effect on Immature Mouse Testicular Tissues, Vitrified-Thawed With Different Cryoprotectant Media
| Author | Mohammadreza Gholami | en |
| Author | Ghasem Saki | en |
| Author | Masoud Hemadi | en |
| Author | Ali Khodadadi | en |
| Author | Javad Mohammadiasl | en |
| Orcid | Ghasem Saki [0000-0001-5268-8407] | en |
| Issued Date | 2015-06-01 | en |
| Abstract | Background: Freezing of the immature testis tissue and then its transplantation can be considered a major step in fertility preservation for young boys with cancer. Objectives: The objective of this study was to investigate the antioxidant effects of melatonin on histological structure of seminiferous tubules that were vitrified-thawed with different cryoprotectant media. Materials and Methods: Male mice were randomly assigned to one of 10 experimental groups. Testes of 6-day-old BALB/c male mice pups were extracted and vitrified-thawed with different vitrification media with melatonin and without melatonin. Then, their testes were stained with haematoxylin-eosin. Results: The result showed that supplementation of glycerol based media with melatonin has reduced injury to histological structure of seminiferous tubules in the 6-day-old male BALB/c mice pups testes (P = 0.004). Conclusions: It seem that melatonin due to its antioxidant properties, if added to glycerol based media, reduces the harmful effects of freezing-thawing process, and protects seminiferous tubules histological structure from oxidative injury. | en |
| DOI | https://doi.org/10.5812/jjhr.28704v2 | en |
| Keyword | Mouse | en |
| Keyword | Testis | en |
| Keyword | Melatonin | en |
| Keyword | Vitrification | en |
| Publisher | Brieflands | en |
| Title | Melatonin Effect on Immature Mouse Testicular Tissues, Vitrified-Thawed With Different Cryoprotectant Media | en |
| Type | Research Article | en |
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