Jentashapir Journal of Cellular and Molecular Biology

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In Collaboration with Ahvaz Jundishapur University of Medical Sciences

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Jentashapir Journal of Cellular and Molecular Biology, JJCMB, publishes original research papers, reviews, meta-analyses, short communications, mini-reviews, commentaries, letter to editors, and case reports in the fields of cellular and molecular biology which demonstrate new advancements and novel results. Additionally, JJCMB publishes all studies in basic, clinical and translational studies. This journal also welcomes studies which focus on new cellular and molecular techniques which promote prognosis, diagnosis, and early diagnosis of human diseases and disorders.

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1) Double-Blind Peer Review System

2) Open Peer Review (since Aug 2019), Show List of All Published Reviewers' Comments

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Now showing 1 - 20 of 500

The Effect of Eight Weeks of Moderate-Intensity Interval Training on Uncarboxylated Osteocalcin Levels and Insulin Resistance Markers in Obese Women
(Brieflands, 2024-12-31) Mostafa Fakhrealali; Keyvan Hejazi; Hamid Marafeti
Background: Osteocalcin (OC) carboxylation functions as a hormone in glucose metabolism, enhancing insulin secretion, sensitivity, and energy consumption. Objectives: This study aimed to examine the effect of eight weeks of moderate-intensity interval training on uncarboxylated OC levels and insulin resistance markers in obese women. Methods: This semi-experimental study included 20 obese women aged 35 to 45, who were randomly divided into two groups: An experimental group (10 participants) and a control group (10 participants). The moderate-intensity interval training program lasted eight weeks, with three sessions per week, each lasting 20 to 40 minutes, at an intensity of 50% to 75% of maximum heart rate. A one-way repeated measures analysis of variance was used to assess intra-group and inter-group changes. Results: The moderate-intensity interval training program resulted in significant reductions in weight, Body Mass Index (BMI), waist-to-hip ratio, insulin levels, and the Insulin Resistance Index. Additionally, uncarboxylated OC levels and maximal oxygen consumption significantly increased by the end of the study period. Time interaction changes within the group were statistically significant for waist-to-hip ratio, glucose, insulin, insulin resistance, and maximal oxygen consumption. Conclusions: Moderate-intensity interval training increased uncarboxylated OC levels and decreased the Insulin Resistance Index in obese women. These findings suggest that moderate-intensity interval training can be recommended as a preventive or therapeutic strategy for managing obesity and potential metabolic diseases associated with obesity.
Unveiling the Cytotoxic and Inhibitory Effects of Ferula gummosa Gum on A-375 Skin Cancer Cells: An in Vitro Study
(Brieflands, 2024-12-31) Fatemeh Eizadifard; Majid Tafrihi
Background: Ferula gummosa Boiss. is a widely recognized species native to Iran, primarily found in the northern and northeastern regions. It has long been valued for its medicinal properties, particularly in traditional medicine, where its gum is used to treat various gastrointestinal disorders and infections. Objectives: However, despite its established benefits, there is a significant research gap regarding its potential anti-cancer properties, specifically against skin cancer cells. Methods: This study aimed to assess the cytotoxic and inhibitory effects of the gum of F. gummosa on the A-375 cell line using several assays, including MTT, clonogenic, acridine orange/ethidium bromide (AO/EtBr) staining, DNA degradation, and in vitro wound-healing experiments. Results: The findings revealed that the gum exhibited notable cytotoxicity on A-375 cells, achieving a 50% inhibitory concentration (IC50) at 8 µg/mL after 48 hours of treatment (compared to control cells) (P < 0.01). The observed DNA degradation pattern suggested a reduction in cell viability, likely due to apoptosis induction. Microscopic examinations showed nuclear condensation and a significant suppression of colony formation in A-375 cells treated with 8 and 10 μg/mL concentrations of the gum, compared to untreated cells. Additionally, wound-healing assessments demonstrated the gum’s ability to inhibit cell migration in contrast to the untreated group. Conclusions: These findings suggest that F. gummosa exhibits significant inhibitory effects on melanoma cancer cells, making it a promising candidate for further investigation.
Increasing the Anaerobic Capacity Resulting from the Improvement of Buffering and Hypoxia Indicators in Healthy Men
(Brieflands, 2024-12-31) Samira Nasiri; Ebrahim Banitalebi; Mohammad Faramarzi
Background: Increasing anaerobic capacity is a crucial factor in enhancing performance in strength sports. Objectives: This study investigated the effect of 10 weeks of weightlifting training with increased respiratory dead space on buffering capacity and anaerobic capacity in healthy men. Methods: Eighteen male weightlifters (mean age: 28.2 ± 3.02 years, BMI: 24.27 ± 1.34 kg/m²) were selected through convenience sampling and randomly assigned to two training groups: With and without masks. Both groups performed selected weightlifting exercises three times per week for 10 weeks at 80% of their one-repetition maximum. The mask group trained using a mask and tube to increase respiratory dead space throughout the training period. The Borg scale was used to measure perceived effort, and the Wingate test assessed anaerobic power and Fatigue Index (FI). Blood samples were taken to measure lactate, CO₂, and hypoxia-inducible factor 1-alpha (HIF-1α) levels. Data analysis was conducted using ANOVA with repeated measures (P ≤ 0.05). Results: Ten weeks of weightlifting training with a mask led to a significant increase in bicarbonate levels (P = 0.029), lactate (P ≤ 0.001), FI (P ≤ 0.001), and rating of perceived exertion (RPE) (P = 0.041) compared to the group without a mask. There was no significant difference between the groups in HIF-1α levels (P = 0.079) or anaerobic power (P = 0.534). However, the percentage changes were greater in the mask group (anaerobic power = 5.9%, HIF-1α = 1.97%) compared to the group without a mask (anaerobic power = -0.9%, HIF-1α = 1.07%). Conclusions: Using the strategy of increasing respiratory dead space during weightlifting training has proven beneficial for enhancing buffering capacity and anaerobic power.
Investigating HOTAIR Polymorphisms as a Potential Diagnostic Marker for Type 2 Diabetes in an Iraqi Population
(Brieflands, 2024-12-31) Zainab Hadi Talib; Amir Jalali; Abbass Haydar
Background: Diabetes mellitus type 2 (T2DM) is a chronic condition characterized by insulin resistance, affecting approximately 530 million adults globally, with a prevalence of 10.5% among individuals aged 20 to 79. In Iraq, the prevalence is significantly higher at 13.9%. Objectives: This study examines the relationship between long noncoding RNA HOX transcript antisense RNA (HOTAIR) polymorphisms and T2DM risk, investigating HOTAIR's potential as a diagnostic marker. Methods: Blood samples were collected from 28 T2DM patients and 20 healthy controls, with physiological parameters measured. HOTAIR plasma levels were assessed using quantitative real-time (qRT)-PCR, and single-nucleotide polymorphisms rs12826786 and rs1899663 were analyzed through amplification refractory mutation system (ARMS)-PCR. Results: HOTAIR expression was found to be 6.6 times higher in T2DM patients compared to controls, suggesting its involvement in T2DM pathophysiology. Genotype distributions adhered to Hardy-Weinberg equilibrium, with the rs12826786 C allele appearing protective against T2DM, while rs1899663 showed no significant association. Statistical analysis identified a significant relationship between the rs12826786 genotype and body mass index (BMI), though other diabetes-related metrics did not show significant results. Conclusions: The findings suggest that elevated HOTAIR expression may play a role in T2DM, highlighting the need for further investigation into these associations and their potential implications for diagnosis and risk assessment.
The results of Pars Plana Vitrectomy in patients with Intraocular Foreign Body
(Brieflands, 2012-06-30)
Background: To identify the clinical features in eyes with intraocular foreign bodies (IOFBs) and evaluate the results of surgical management in these cases. Material and methods: In a descriptive retrospective study, the records of 32 eyes of 32 patients with IOFBs were reviewed. All eyes underwent pars plana vitrectomy between April 2001 and February 2012. The IOFBs were removed either with intraocular forceps or magnetic extraction. Results: All patients were men with the mean age of 30.26 years. The average follows up period was 25.16 months. The IOFBS was ferromagnetic in 25 (70.5%). The IOFBs were extracted with external magnet in 17 eyes (50%) and with intraocular forceps in 12 eyes (35.2%). Macular pucker and scar were present in six eyes (18.7%). Final visual acuity was 20/40 or better in 7 eyes (21.9%) and 20/200 or better in 16 eyes (50%). There were no significant relationship between the final visual acuity of 20/200 or better and the site (P=0.59), size (P=0.53), type of IOFBs (P=0.39), and time interval between trauma and surgery (P=0.34).
Green Copper Carbonate Nanoparticles Produced by the Ureolytic Fungus Alternaria Species Strain ccf7 and Their Antibacterial Activity
(Brieflands, 2023-06-30) Morahem Ashengroph; Zahra Rabiei
Background: Copper carbonate nanoparticles have several applications in the fields of pigments, insecticides, and fungicides. They are also used as catalysts in chemical processes and crude oil desulfurization. Fungi can biosynthesize metal nanoparticles due to their high tolerance, extracellular synthesis, simplicity of extraction, and large-scale exploitation. Objectives: This study aimed to investigate the potential of fungal isolates (which are resistant to copper chloride with urease activity) as biocatalysts for the synthesis of copper carbonate nanoparticles. This approach was considered due to the advantages of using fungal isolates in nanoparticle biosynthesis. Methods: In a PDA culture medium with 25 mM copper chloride, an enrichment culture was used to isolate copper-resistant fungal isolates. Fungal isolates’ urease enzyme was qualitatively assessed using 2% urea agar-based culture media. Studies on the synthesis of copper carbonate nanoparticles and the effect of different parameters on the synthesis of these nanoparticles were conducted using a mycelium-free supernatant strategy. Field emission scanning electron microscope (FESEM), dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDX), and Fourier transform infrared spectroscopy (FTIR) studies were used to determine the properties of calcium carbonate nanoparticles. The selected fungal isolate was identified using macroscopic and microscopic characteristics, as well as molecular analysis using amplification of the ITS1-5.8S-ITS2 gene sequences. Results: Alternaria species strain ccf7 (GenBank accession number OP242500) was chosen as the superior strain for copper carbonate nanoparticle synthesis tests based on the pattern of resistance to copper chloride salt and the qualitative assessment of urease activity. Based on the findings of the electron microscope studies, spherical copper carbonate nanoparticles with an average size of 66.7 nm were synthesized after 24 hours of incubation at the optimal concentration of 45mM copper chloride, temperature of 25°C, and shaker speed of 100 rpm. The distribution of the produced nanoparticles was appropriate, as indicated by a polydispersity index (PDI) of 0.25. The strongest inhibitory impact of these copper carbonate nanoparticles was against Pseudomonas aeruginosa, with an average inhibition of 31 mm at a concentration of 50 mg/L, according to the results of their antibacterial activities. Conclusions: For the first time, the synthesis and development of a green approach for the fabrication of copper carbonate nanoparticles using the genus Alternaria have been proposed in this study.
Study of Erythrocyte Sedimentation Rate) ESR) in patients referred to Golestan hospital of Ahvaz in 2009
(Brieflands, 2011-06-30)
Background: One of the most common tests performed in the hematology laboratories is erythrocyte sedimentation rate (ESR) that for monitoring infections, inflammatory and cancers diseases. The aim of this study was investigation of ESR in patients referred to Golestan hospital, Ahvaz. Material and methods: This descriptive and cross-sectional study was done in second half of 1388 on outpatient and hospitalized in different wards of the Golestan hospital, Ahvaz, and ESR was tested by Westergrene method. Data were analyzed by SPSS and using T test. Results: In the present study, mean of Erythrocyte Sedimentation Rate was in males and female, 34 / 5 12/5 and 33/9 12 respectively , that this difference was not statistically significant (P>0/05) , mean of age was in males and female, 28/1 13,and 26/9 12/1 respectively, the difference was not statistically significant (P>0/05). There were 51 % males and 49% female.38%of patients in outpatient and 62% in different wards of, the Golestan Hospital, were hospitalized.
The results of Pars Plana Vitrectomy in patients with Intraocular Foreign Body
(Brieflands, 2012-06-30)
Background: To identify the clinical features in eyes with intraocular foreign bodies (IOFBs) and evaluate the results of surgical management in these cases. Material and methods: In a descriptive retrospective study, the records of 32 eyes of 32 patients with IOFBs were reviewed. All eyes underwent pars plana vitrectomy between April 2001 and February 2012. The IOFBs were removed either with intraocular forceps or magnetic extraction. Results: All patients were men with the mean age of 30.26 years. The average follows up period was 25.16 months. The IOFBS was ferromagnetic in 25 (70.5%). The IOFBs were extracted with external magnet in 17 eyes (50%) and with intraocular forceps in 12 eyes (35.2%). Macular pucker and scar were present in six eyes (18.7%). Final visual acuity was 20/40 or better in 7 eyes (21.9%) and 20/200 or better in 16 eyes (50%). There were no significant relationship between the final visual acuity of 20/200 or better and the site (P=0.59), size (P=0.53), type of IOFBs (P=0.39), and time interval between trauma and surgery (P=0.34).
Personal, Familial, and Social Factors Contributing to Addiction Relapse, Ahvaz, Iran
(Brieflands, 2015-06-01) Sedigheh Fayazi; Dariush Rokhafroz; Mahin Gheibizadeh; Ashrafalsadat Hakim; Neda Sayadi
Background: Drug addiction is deemed one of the gravest threats to society. Objectives: The objective of this study was to determine what factors (personal, familial, or social) are correlated with addiction relapse. Patients and Methods: In this descriptive study, 146 addicts referring to addiction treatment centers in the Iranian city of Ahvaz were selected via purposive and non-randomized sampling. The study tool was a researcher-made questionnaire. Descriptive statistics and SPSS software were used for data analysis. Results: The results showed that 46.1% of the participants aged between 20 and 30 years. All the subjects had at least one attempt at quitting drug abuse. Single individuals comprised 52.9% of the study population. The most significant physical factors were lack of appetite (23.9%), numbness and pins and needles (23.3%), and bone pain (22.4%), while the most significant mental factor was loneliness (44%). Concerning the social factors, association with addicted and misleading friends (35.2%) had the utmost importance. Furthermore, lack of a permanent job (43%) and absence of appropriate family relationships (32%), respectively, constituted the most important factors among the career and familial factors. Conclusions: Our results showed that many personal, familial, and social factors play a role in addiction relapse. The high prevalence of return to addiction necessitates further strategies for the more optimal control of these factors.
The relationship between communication skills and marital satisfaction among married students of Psychology and Counseling
(Brieflands, 2010-06-30)
Background: The purpose of this study was to investigate the relationship between communication skills and marital satisfaction in married students of Psychology and Counseling, studying in Ahvaz Shahid Chamran University and Ahvaz branch of Azad university . Material and methods: the sample for this study consists of 75 married students of Clinical Psychology and Counseling, studying in Ahvaz Shahid Chamran University and Ahvaz branch of Azad University , selected by Convenient sampling. Mean and Standard Deviation (SD) of the age of subjects were 27.33 and 6.31 respectively. The data was gathered by using Enrich marital satisfaction questionnaire and Queendom communication skills Inventory. Results: There are significant positive correlation between communication skills and its subscales with : marital satisfaction.
Prevalence of Comorbid Psychiatric Disorders in Children and Adolescents With Attention Deficit Hyperactivity Disorder
(Brieflands, 2015-08-01) Forough Riahi; Maryam Izadi-Mazidi; Soheila Mohammadi Abdi
Background: Attention deficit hyperactivity disorder (ADHD) is often associated with other psychological problems. Objectives: The present study aimed to study the prevalence of comorbid psychiatric disorders in children and adolescents with ADHD who admitted to Golestan Hospital in Ahvaz. Patients and Methods: This was a descriptive/analytic cross-sectional study carried out on 118 outpatient children and adolescents who were selected by convenient sampling. The data were collected using the questionnaire, designed by authors, and were analyzed through descriptive statistics and chi-square test. Results: The prevalence of comorbid disorders were as follows: anxiety disorders (48.3%); depression (20.33%); bipolar disorder (17.79%); obsessive-compulsive (47.45%); tic and tourette (35.59%), oppositional defiant disorder (43.22%); conduct disorder (11.01%); urinary incontinence (58.47%); communication disorder (9.32%); and learning disorder (21.18%). There was no significant difference between females and males with respect to the prevalence of comorbid disorders. Conclusions: Similar to previous studies, we found some comorbid psychiatric disorders with ADHD. The treatment of the disorder can be improved, by more attention to comorbid psychiatric disorders, early diagnosis of them, and using distinct and specific treatment for everyone.
Design and test a model of the relationship between negative life events and mental health with resiliency and social support mediation in female medical sciences students at jundishapour university of Ahvaz
(Brieflands, 2014-02-28) Fatemeh Nadi Najafabadi; Akram Nazari Chegeni; Soodabeh Bassaknejad
Background: The purpose of this study examined the relationship between negative life events and mental health with social support and resiliency mediation in female medical sciences students at Jundishapour University of Ahvaz.
Association of Rose angina with cardiovascular risk factors among men and women: a population-based study
(Brieflands, 2014-10-31) Karamatollah Rahmanian
In this cross-sectional study, persons with 30 years old or older were participated in Jahrom. Rose angina was assessed with Rose questionnaire. Fasting blood lipids and sugar, blood pressure, weight and height were measured. Data were analyzed by chi squared and logistic regression tests. Results: The prevalence of Rose angina was 21.1%. Women had Rose angina 12.4% more than men (26.5% in women, 14.3% in men; p
Biotinidase, as a Convenient Diagnostic and Prognostic Marker for Breast Cancer
(Brieflands, 2015-02-01) Nader Saki; Soheila Nikakhlagh; Gholamabbas Dinarvand
According to the definition by the National Institutes of Health of USA, a biological marker (biomarker) is a characteristic that is objectively measured and evaluated, as an indicator of normal biological processes, pathogenic processes or pharmacological responses, to a therapeutic agent. Several studies have reported that a decreased regulating gene expression of biotinidase (BTD) is associated with breast cancer. On the basis of these evidences, we hypothesize that in the serum of breast cancer patients, with different pathological grades, reductions in serum BTD enzyme activities, there are significant differences. The use of this marker, as a diagnostic and prognostic marker for breast cancer, is a promising perspective for the clinical field.
Associations Between the TNF-Alpha-238 Gene (rs361625) Polymorphisms and Lung Cancer: A Meta-analysis
(Brieflands, 2023-03-31) Sanaz Pashapour; Sahar Saki; Elham Sadat Afraz; Yeganeh Hamidi; Leila Najd Hassan Bonab
Context: The relationship between polymorphisms in the location of the cytokine tumor necrosis factor (TNF-α) and lung cancer has been investigated in many studies. Accordingly, the present meta-analysis study focused on the relationship between the TNF-α-238 gene polymorphisms and lung cancer. Methods: Articles were collected from Google Scholar, Scopus, and PubMed electronic databases until 2022. The articles were searched based on the keywords “Lung cancer,” “238 Gene”, and “tumor necrosis factor.” The articles were selected based on the PRISMA flow diagram. Results: There was no bias in this study research. Two except for two studies were significantly different, while no significant difference was found in the other studies. However, the results of the final Overall OR with a value of (0.66; 1.88) OR = 1.11 indicate that the additive model in the TNF-α-238 (rs361525) SNP increases the risk of lung cancer in the random model (P < 0.01). Conclusions: The results of this meta-analysis study show the relationship between TNF-a-238 and lung cancer. The TNF-a-238 polymorphism increases the risk allele, and TNF-a-238 with an OR = 1.11 has an additive effect on lung cancer development and increases the risk of lung cancer.
Status of organizational learning among faculty members of Ahvaz Jundishapur University of Medical Sciences in 2011
(Brieflands, 2013-06-30)
This article does not have abstract.
Skin as a Psychoneuroendocrine Immunology Microcosm: The New Frontier of Low Dose Therapy With Cytokines and Growth Factors in the Systemic Treatment of Chronic Autoimmune Inflammatory Diseases in Dermatology
(Brieflands, 2016-04-01) Torello Lotti; Tatiana Rivkina; Massimo Fioranelli
In recent years, the central role of signaling molecules, such as hormones, cytokines and growth factors, has become evident in both physiological and pathological processes; these signaling molecules are the main regulating effectors of whole body biological functions, in accordance with the guiding principle of psychoneuroendocrine immunology (PNEI).
A case report of diaphragmatic eventration in a premature newborn
(Brieflands, 2011-06-30)
Background: Diaphragm of eventration is a defect of whole or a part of diaphragm muscle and replacing it with fibroplastic tissue. The incidence of eventration is uncertain. The article aims to report a newborn with congenital diaphragmatic eventration. Case report: The case is a dysmorphic male premature newborn which born at 33 gestational age. Due to sever respiratory distress the patient admitted immediately in NICU and underwent associated ventilation. Despite thoracotomy and also exploratory surgery, he died after thirty days. Conclusion: We as health care givers have responsibility to inform people about prevention of genetic abnormalities and importance of genetic consoling before decision for having a child.
Designing an Educational Application of Parental-Mediated Intervention and Its Effectiveness to Promote Reading Skills Among Slow-Paced Students with Down Syndrome
(Brieflands, 2017-02-28) Kosar Bereyhi; Shahla Pakdaman; Gholamali Afrooz
Objectives: This study aimed to design an educational application of parental-mediated intervention and its effectiveness to promote reading skills in students with Down syndrome. Methods: This applied semi-experimental study is a pre-test- and post-test project, follow-up with the test and control groups which was conducted on twenty slow-paced students with Down syndrome in the range of 5 to 12 years old. Patients were randomly selected and classify into two groups; test and control. Wechsler IQ test, TOLD test and peabody picture vocabulary test (PPVT) were performed for students in the pre-test however; TOLD test was conducted as the post-test and a half month at 15-day after follow-up stage. Results: results showed α > 0.001 for reading skills between test and control groups; however the difference is remained sustainable in follow-up stage. Conclusions: Education with new educational technologies that focused on software may be helpful for children with Down syndrome and should be seriously considered. Family- centered parental-mediated intervention in order to promote reading skills application can be used for teaching children, families and educators.
Determination of the Antibacterial and Apoptotic Effects of Carvacrol and Anti-cancer Peptide
(Brieflands, 2022-12-31) Mona Ghods Elahi; Malak Hekmati; Davoud Esmaeili; Parisa Ziararti; Mohamad Yousefi
Background: Treating gastric cancer and antibacterial remains a major challenge. There have been many reports about the positive effects of carvacrol and anti-cancer peptides contributing to cancer inhibition and antibacterial activities. Objectives: This study aimed to determine antibacterial and anti-cancer effects of modified carvacrol against AGS gastric cancer cell line by adopting the flow cytometry technique. Methods: The treatment of cells with modified carvacrol containing anti-cancer peptide was used to evaluate the apoptosis effect against the AGS cell line. The treatment of AGS cells was performed by adopting flow cytometry in order to evaluate the apoptosis. Disc diffusion and MIC methods were used to determine the antibacterial effects. Results: The results showed that cells treated with carvacrol and anti-cancer peptide at a concentration of 0.125 µg/mL induced a 31-fold apoptotic effect. Phosphate buffered saline was used as the control group for the treatment that induced a 63% apoptotic effect on the AGS cell line. The results of antibacterial activity suggested that the modified carvacrol had antibacterial properties against Staphylococcus aureus (MIC = 6 µg/mL, Growth inhibition zone with diameter of 20mm) and Escherichia coli (12 µg/mL, Growth inhibition zone with diameter 16). The results also revealed that the minimum inhibitory concentration of the compound was 6 µg/mL for S. aureus, while this value was 12 µg/mL for E. coli. Conclusions: Since the anti-cancer properties of modified carvacrol mixture with anti-cancer peptide induced 1.25 times more than phosphate-buffered saline, the above combination may have been used to treat and induce apoptotic activity against gastric cancer cell lines.

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