The Effect of Aspalathin on Levels of Sugar and Lipids in Streptozotocin-Induced Diabetic and Normal Rats

Abstract

Background: Flavonoids have been reported as mammalian alpha-amylase inhibitors, a property which could be useful in the management of postprandial hyperglycemia in diabetes and its related disorders. Objectives: In the present study the inhibitory effect of aspalathin as a flavonoid on alpha amylase activity and levels of sugar and lipids in rats, has been investigated. Methods: In this experimental study, type inhibition of aspalatin on amylase and in the part of in vivo, the effect of aspalathin orally doses 5, 10, 20 and 40 mg/kg body weight on sugar and lipids levels was tested in a streptozotocin-induced model of diabetes and normal rats. The data were analyzed by one-sample Kolmogrov-Smirnov, Levene and ANOVA tests through SPSS version 22. Results: The results showed that aspalathin is a competitive inhibitor for alpha amylase with Ki = 37.0 μM. In both diabetic and normal groups in all doses nearly dose dependent manner reduced blood glucose levels and beneficial effect on dyslipidemia were observed in diabetic rats, as well as reduction of disturbing consequences of diabetes such as high urine volume and water intake. Aspalathin was observed to have a weight loss-inductive effect, alongside with a reduction in food intake. Conclusions: It seems that, this compound could be proposed as an antihyperglycemic and antihyperlipidemic agent in diabetes and potential therapeutic in obesity.