The Complexity of the Identification of <i>Burkholderia cepacia</i> Strain Which Caused Septicemia
| Author | Lan Anh Thi Bui | en |
| Author | Irina Zakharova | en |
| Author | Ivan Shpak | en |
| Author | Natalya Teteryatnikova | en |
| Author | Dimitriy Ustinov | en |
| Author | Yulia Kuzyutina | en |
| Author | Ha Ngoc Nguyen | en |
| Author | Dmitry Viktorov | en |
| Issued Date | 2018-11-30 | en |
| Abstract | Background: The correct identifying of pathogenic Burkholderia spp. using available commercial biochemical systems is a certain problem due to metabolic plasticity and variable enzymatic profile of isolates. Objectives: The current study aimed at using specific PCR and conventional multi-locus sequence typing (MLST) scheme to confirm the uncertain identification results for Burkholderia cepacia clinical isolate. Methods: Multilocus species-specific PCR and MLST profiling of high-throughput sequencing data have been used to clarify the varied results of biochemical identification of the strain. Results: The strain isolated from a patient with septicemia was initially identified as B. pseudomallei by Vitek 2 GN system but has an uncharacteristic antimicrobial resistance pattern and colony morphology. A species-specific multilocus PCR and whole-genome sequence profiling, according to the MLST scheme, allowed to identify an isolate as B. cepacia. Conclusions: The obtained results demonstrate the preference of molecular tests for correct identifying of pathogenic Burkholderia, considering that misidentification of those may lead to improper treatment or increase of biosafety risk. | en |
| DOI | https://doi.org/10.5812/jjm.82834 | en |
| Keyword | <i>Burkholderia cepacia</i> | en |
| Keyword | <i>B. pseudomallei</i> | en |
| Keyword | Sepsis | en |
| Keyword | PCR | en |
| Keyword | Whole Genome Sequencing | en |
| Keyword | Molecular Typing | en |
| Publisher | Brieflands | en |
| Title | The Complexity of the Identification of <i>Burkholderia cepacia</i> Strain Which Caused Septicemia | en |
| Type | Brief Report | en |
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