Prevalence of Plasmid-Mediated Quinolone Resistance (PMQR) Determinants Among Extended Spectrum Beta-Lactamases (ESBL)-Producing Isolates of Escherichia coli and Klebsiella pneumoniae in Aleppo, Syria
| Author | Omar Alheib | en |
| Author | Rawaa Al Kayali | en |
| Author | M. Yaser Abajy | en |
| Issued Date | 2015-07-01 | en |
| Abstract | Background: Recently, several plasmid-mediated quinolone resistance (PMQR) genes conferring low levels of quinolone resistance have been discovered. Objectives: The aim of the present study is to determine the prevalence of plasmid-mediated quinolone resistance genes in a collection of ESBL-producing isolates of E. coli and K. pneumonia in Aleppo, Syria. Materials and Methods: Here, to evaluate the prevalence of PMQR genes at Aleppo University hospitals in Syria, 123 extended spectrum beta-lactamases (ESBL)-producing isolates of Escherichia coli and Klebsiella pneumoniae from the hospitals were selected for screening based on ciprofloxacin resistance. Five PMQR genes [qnrA, qnrB, qnrS, aac (6’)-Ib, and qepA] were screened by simplex PCR, and the aac (6’)-Ib-positive PCR products were digested with BtsCI to detect the aac (6’)-Ib-cr variant. Results: Of the 123 isolates, 103 (83.73%) had one of the five PMQR genes, including 83 (83.83%) of the 99 E. coli strains and 20 (86.95%) of the 23 K. pneumoniae strains. Conclusions: The most common qnr gene was qnrB, and none of the isolates carried qnrA or qepA. The aac (6’)-Ib-cr variant was the most prevalent PMQR gene, and it was associated with the prevalence of ciprofloxacin resistance in our ESBL-producing isolates. | en |
| DOI | https://doi.org/10.5812/archcid.20631 | en |
| Keyword | QepA Protein | en |
| Keyword | Beta-Lactamase | en |
| Keyword | Ciprofloxacin | en |
| Keyword | <i>Escherichia coli</i> | en |
| Keyword | <i>Klebsiella pneumoniae</i> | en |
| Publisher | Brieflands | en |
| Title | Prevalence of Plasmid-Mediated Quinolone Resistance (PMQR) Determinants Among Extended Spectrum Beta-Lactamases (ESBL)-Producing Isolates of Escherichia coli and Klebsiella pneumoniae in Aleppo, Syria | en |
| Type | Brief Report | en |
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