Effect of High-Intensity Interval Training on Apoptotic Gene Expression in Rat Myocardial Tissue
| Author | Hassan Pourrazi | en |
| Author | Masoud Asgharpour-Arshad | en |
| Author | Farhad Gholami | en |
| Author | Soheila Abbasi | en |
| Orcid | Hassan Pourrazi [0000-0003-2088-9671] | en |
| Orcid | Masoud Asgharpour-Arshad [0000-0001-9597-0816] | en |
| Orcid | Farhad Gholami [0000-0002-9037-2259] | en |
| Orcid | Soheila Abbasi [0000-0002-7068-8213] | en |
| Issued Date | 2020-04-30 | en |
| Abstract | Background: Excessive apoptosis in the myocardium contributes to the pathogenesis of heart disease. Evidence indicates that high-intensity interval training (HIIT) can alter myocardial apoptosis-related signaling. Objectives: The purpose of the study was to investigate the effect of 12 weeks of HIIT on some indices of apoptosis, including Bcl-2, Bax, and caspase-3 in the myocardial tissue of male rats. Methods: Thirty male Wistar rats (age range: 8 - 10 weeks) were randomized into three groups, including sham (n = 10), control (n = 10), and HIIT (n = 10). The experimental group underwent HIIT consisting of 2 - 8 repetitions of 4-min high-intensity intervals (85% - 90% peak speed) interspersed with low-intensity intervals (45% - 50% peak speed), performed five times/week over 12 weeks. Forty-eight hours after the last training session, animals’ hearts were removed. The gene expressions of Bcl-2, Bax, and caspase-3 were analyzed by the RT-PCR method. The independent t-test was used for statistical analysis (P < 0.05). Results: The gene expressions of Bcl-2 and Bcl-2/Bax ratio were higher in the training group than in the control group (P = 0.001 and P = 0.002, respectively). Regarding Bax and caspase-3 gene expression, although the mean levels were lower in the exercise group than in the control group, the differences were statistically insignificant (P = 0.57 and P = 0.27, respectively). Conclusions: Overall, it seems that HIIT significantly increases the expression of anti-apoptotic indicator Bcl-2 and the ratio of Bcl-2/Bax in the myocardium of male rats. However, further studies are needed to show the effects of HIIT on myocardial apoptosis. | en |
| DOI | https://doi.org/10.5812/gct.101963 | en |
| Keyword | High-Intensity Interval Training | en |
| Keyword | Apoptosis | en |
| Keyword | Myocardium | en |
| Keyword | Apoptotic Genes | en |
| Publisher | Brieflands | en |
| Title | Effect of High-Intensity Interval Training on Apoptotic Gene Expression in Rat Myocardial Tissue | en |
| Type | Research Article | en |
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