Assessing the Expression Levels of miR-1307-3p and miR-3613-5p in COVID-19 Patients

AuthorRahil Ghanbarnasab Behbahanien
AuthorMojtaba Rastien
AuthorNasrin Rastegarvanden
AuthorMehdi Parsanahaden
AuthorAmir Danyaeien
AuthorNiloofar Neisien
AuthorRoya Pirmoradien
AuthorAli Teimoorien
OrcidRahil Ghanbarnasab Behbahani [0000-0003-0657-9191]en
OrcidMojtaba Rasti [0000-0002-9622-4839]en
OrcidAmir Danyaei [0000-0003-4855-0514]en
OrcidNiloofar Neisi [0000-0001-8224-4890]en
Issued Date2025-04-30en
AbstractBackground: MicroRNAs (miRNAs) regulate the immune response to infectious diseases. They exhibit alterations during the early stages of disease development, even before the pathogen is detectable. Objectives: The objective of this study was to analyze the expression levels of miR-1307-3p and miR-3613-5p at the onset of COVID-19, aiming to identify miRNAs that could function as early predictive biomarkers. Methods: This case-control study analyzed the expression levels of miR-1307-3p and miR-3613-5p at the onset of COVID-19 to identify early predictive biomarkers. Plasma miRNAs from 26 COVID-19 patients hospitalized in the ICU ward were sequenced and compared to a healthy control group of 26 individuals. Additionally, plasma biomarkers associated with the immune system were evaluated. Total RNA was extracted using an RNX solution and subjected to cDNA synthesis with a specific loop sequence for each miRNA. The samples were subsequently analyzed using relative real-time PCR. Results: RT-qPCR analysis revealed a significant reduction in the detection rate of miR-1307-3p (P < 0.0001) in the patient group compared to the control group. Conversely, no significant differences were observed in the levels of miR-3613-5p between the patient and control groups. Conclusions: miR-1307-3p shows potential as a biomarker for the diagnosis of COVID-19.en
DOIhttps://doi.org/10.5812/archcid-143716en
KeywordCOVID-19 Patientsen
KeywordMicroRNAsen
KeywordGene Expressionen
PublisherBrieflandsen
TitleAssessing the Expression Levels of miR-1307-3p and miR-3613-5p in COVID-19 Patientsen
TypeResearch Articleen

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
archcid-20-1-143716-publish-pdf.pdf
Size:
205.27 KB
Format:
Adobe Portable Document Format
Description:
Article/s PDF