Assessing the Expression Levels of miR-1307-3p and miR-3613-5p in COVID-19 Patients
| Author | Rahil Ghanbarnasab Behbahani | en |
| Author | Mojtaba Rasti | en |
| Author | Nasrin Rastegarvand | en |
| Author | Mehdi Parsanahad | en |
| Author | Amir Danyaei | en |
| Author | Niloofar Neisi | en |
| Author | Roya Pirmoradi | en |
| Author | Ali Teimoori | en |
| Orcid | Rahil Ghanbarnasab Behbahani [0000-0003-0657-9191] | en |
| Orcid | Mojtaba Rasti [0000-0002-9622-4839] | en |
| Orcid | Amir Danyaei [0000-0003-4855-0514] | en |
| Orcid | Niloofar Neisi [0000-0001-8224-4890] | en |
| Issued Date | 2025-04-30 | en |
| Abstract | Background: MicroRNAs (miRNAs) regulate the immune response to infectious diseases. They exhibit alterations during the early stages of disease development, even before the pathogen is detectable. Objectives: The objective of this study was to analyze the expression levels of miR-1307-3p and miR-3613-5p at the onset of COVID-19, aiming to identify miRNAs that could function as early predictive biomarkers. Methods: This case-control study analyzed the expression levels of miR-1307-3p and miR-3613-5p at the onset of COVID-19 to identify early predictive biomarkers. Plasma miRNAs from 26 COVID-19 patients hospitalized in the ICU ward were sequenced and compared to a healthy control group of 26 individuals. Additionally, plasma biomarkers associated with the immune system were evaluated. Total RNA was extracted using an RNX solution and subjected to cDNA synthesis with a specific loop sequence for each miRNA. The samples were subsequently analyzed using relative real-time PCR. Results: RT-qPCR analysis revealed a significant reduction in the detection rate of miR-1307-3p (P < 0.0001) in the patient group compared to the control group. Conversely, no significant differences were observed in the levels of miR-3613-5p between the patient and control groups. Conclusions: miR-1307-3p shows potential as a biomarker for the diagnosis of COVID-19. | en |
| DOI | https://doi.org/10.5812/archcid-143716 | en |
| Keyword | COVID-19 Patients | en |
| Keyword | MicroRNAs | en |
| Keyword | Gene Expression | en |
| Publisher | Brieflands | en |
| Title | Assessing the Expression Levels of miR-1307-3p and miR-3613-5p in COVID-19 Patients | en |
| Type | Research Article | en |
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