Evaluation of Cellular miR-122 Expression in Association with the Presence of Varicella-Zoster Virus among Central Nervous System Tumors
| Author | Aida Abbasi | en |
| Author | Javid Sadri Nahand | en |
| Author | Mohsen Moghoofei | en |
| Author | Maryam Esghaei | en |
| Author | Davod Javanmard | en |
| Author | Mohammad Hadi Karbalaie Niya | en |
| Author | Farzin Sadeghi | en |
| Author | Farah Bokharaei-Salim | en |
| Author | Mohammad Karimzadeh | en |
| Author | Amirhossein Khodayari | en |
| Author | Hossein Keyvani | en |
| Orcid | Maryam Esghaei [0000-0003-2944-2147] | en |
| Orcid | Mohammad Hadi Karbalaie Niya [0000-0002-1364-5743] | en |
| Orcid | Farzin Sadeghi [0000-0001-8599-3643] | en |
| Orcid | Farah Bokharaei-Salim [0000-0002-5367-0847] | en |
| Orcid | Hossein Keyvani [0000-0001-8054-1939] | en |
| Issued Date | 2022-11-30 | en |
| Abstract | Background: Brain tumors are all primary central nervous system (CNS) tumors with unclear etiologies and viral infections, especially human herpesviruses, which have emerged as a hot topic for comprehensive research. Objectives: The present study aimed at assessing the molecular epidemiology of varicella-zoster virus (VZV) and its association with microRNA 122 (miR-122) expression in CNS tumor samples. Methods: Fresh frozen tissue samples were collected from 60 CNS tumor patients and 45 healthy controls. A nested PCR assay was performed to detect the VZV-DNA. Subsequently, the expression level of miR-122 was evaluated in the CNS tumor tissue samples of patients and the brain tissue samples were obtained from healthy controls, using a real-time PCR assay. Results: Of 60 patients with CNS tumors, 29 were men and 31 were women. VZV-DNA was detected in 13.3% of the CNS tumor tissue specimens. There was no statistically significant association between the presence of VZV-DNA and different types of CNS tumors (P > 0.05). Furthermore, the expression level of miR-122 was significantly downregulated in the CNS tumor tissue samples obtained from the patients compared with those of the healthy controls (P < 0.05). Additionally, the expression level of miR-122 was significantly lower in the VZV-positive tumor samples as compared with those of the VZV-negative tumor samples and the healthy controls. Conclusions: Although VZV plays no direct role in the development of CNS tumors, the virus may affect the biology of CNS tumors by decreasing the expression levels of miR-122, which consequently leads to an increased risk of malignancy. However, the experimental data are not conclusive enough; so, further investigations are needed. | en |
| DOI | https://doi.org/10.5812/ijcm-108497 | en |
| Keyword | Varicella-zoster Virus | en |
| Keyword | Central Nervous System | en |
| Keyword | CNS | en |
| Keyword | Tumor | en |
| Keyword | MicroRNA 122 | en |
| Keyword | miR-122 | en |
| Keyword | Glioblastoma Multiforme | en |
| Publisher | Brieflands | en |
| Title | Evaluation of Cellular miR-122 Expression in Association with the Presence of Varicella-Zoster Virus among Central Nervous System Tumors | en |
| Type | Research Article | en |
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