Enzyme and Thermo Dual-stimuli Responsive DOX Carrier Based on PNIPAM Conjugated Mesoporous Silica
| Author | Seyyed Mostafa Ebrahimi | en |
| Author | Mahdieh Karamat Iradmousa | en |
| Author | Mahtab Rashed | en |
| Author | Yousef Fattahi | en |
| Author | Yalda Hosseinzadeh Ardakani | en |
| Author | Saeed Bahadorikhalili | en |
| Author | Reza Bafkary | en |
| Author | Mohammad Erfan | en |
| Author | Rassoul Dinarvand | en |
| Author | Arash Mahboubi | en |
| Orcid | Arash Mahboubi [0000-0002-5140-8159] | en |
| Issued Date | 2022-12-31 | en |
| Abstract | Background: Stimuli-responsive drug delivery systems have been proven to be a promising strategy to enhance tumor localization, overcome multidrug resistance (MDR), and reduce the side effects of chemotherapy agents. Objectives: In this study, a temperature and redox dual stimuli-responsive system using mesoporous silica nanoparticles (MSNs) for targeted delivery of doxorubicin (DOX) was developed. Methods: Mesoporous silica nanoparticles were capped with poly(N-isopropylacrylamide) (PNIPAM), a thermo-sensitive polymer, with atom transfer radical polymerization (ATRP) method, via disulfide bonds (DOX-MSN-S-S-PNIPAM) to attain a controlled system that releases DOX under glutathione-rich (GSH-rich) environments and temperatures above PNIPAM’s lower critical solution temperature (LCST). Morphological and physicochemical properties of the nanoparticles were indicated using transmission electron microscopy (TEM), dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDS), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and Brunauer-Emmett-Teller (BET). The drug release tests were performed at 25°C and 41°C in the absence and presence of the DTT, and the obtained results confirmed the synergic effect of temperature and reductive agent on a dual responsive release profile with a 73% cumulative release at 41°C and reductive environment during 240 min. Results: The average loaded drug content and encapsulation efficacy were reported as 42% and 29.5% at the drug: nanoparticle ratio of 1.5: 1. In vitro cytotoxicity assays on MCF-7 cell lines indicated significant viability decreased in cells exposed to DOX-MSN-S-S-PNIPAM compared to the free drug (DOX). Conclusions: Based on the results, DOX-MSN-S-S-PNIPAM has shown much more efficiency with stimuli-responsive properties in comparison to DOX on MCF-7 cancer cell lines. | en |
| DOI | https://doi.org/10.5812/ijpr-130474 | en |
| URI | https://brieflands.com/journals/ijpr/articles/130474 | en |
| Keyword | ATRP Polymerization | en |
| Keyword | Drug Delivery | en |
| Keyword | Stimuli-Responsive | en |
| Keyword | Cancer | en |
| Keyword | Nanoparticles | en |
| Publisher | Brieflands | en |
| Title | Enzyme and Thermo Dual-stimuli Responsive DOX Carrier Based on PNIPAM Conjugated Mesoporous Silica | en |
| Type | Research Article | en |