Colloidal Gold Assay, a New Screening Test for Detection of RSV Infection: A Controlled Trial
| Author | Lifang Liu | en |
| Author | Wei Li | en |
| Author | Ran Tao | en |
| Author | Huamei Li | en |
| Author | Shiqiang Shang | en |
| Issued Date | 2019-10-31 | en |
| Abstract | Background: Respiratory syncytial virus (RVS) is the main cause of morbidity and mortality in the pediatric population. Objectives: The objective of the study was to compare the diagnostic accuracies of the colloidal-gold-Genesis and newly developed real-time RT-PCR-IngeniGen with an admitted real-time RT-PCR-HuaRuiAn as the gold standard for respiratory syncytial virus detection. Methods: These three assays were performed on collected specimens. Results: Altogether 258 specimens were studied. Among them, real-time RT-PCR-HuaRuiAn detected the respiratory syncytial virus in 166 samples, while colloidal-gold-Genesis detected 171 samples and real-time RT-PCR-IngeniGen detected 168 samples as positive. The overall sensitivity for the detection of the respiratory syncytial virus in comparison to real-time RT-PCR-HuaRuiAn was 95.2% for colloidal-gold-Genesis and 100% for real-time RT-PCR-IngeniGen. The specificity was 85.9% for colloidal-gold-Genesis and 97.8% for real-time RT-PCR-IngeniGen. The positive likelihood ratio was 6.75 for colloidal-gold-Genesis and 45.45 for real-time RT-PCR-IngeniGen. The negative likelihood ratio was 0.06 for colloidal-gold-Genesis and 0.00 for real-time RT-PCR-IngeniGen. The cycle threshold value of real-time RT-PCR-IngeniGen was higher than that of real-time RT-PCR-HuaRuiAn. Conclusions: Colloidal-gold-Genesis is a good alternative for fast screening of respiratory syncytial virus detection. | en |
| DOI | https://doi.org/10.5812/ijp.89082 | en |
| Keyword | Respiratory Syncytial Virus (RSV) | en |
| Keyword | Real-Time RT-PCR | en |
| Keyword | Colloidal Gold Assay | en |
| Publisher | Brieflands | en |
| Title | Colloidal Gold Assay, a New Screening Test for Detection of RSV Infection: A Controlled Trial | en |
| Type | Brief Report | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- ijp-29-5-89082.pdf
- Size:
- 171.43 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF