Prevalence of Antibiotic Resistance and Distribution of Virulence Factors in the Shiga Toxigenic <i>Escherichia coli</i> Recovered from Hospital Food
| Author | Reza Ranjbar | en |
| Author | Ali Seif | en |
| Author | Farhad Safarpoor Dehkordi | en |
| Orcid | Reza Ranjbar [0000-0001-8593-1514] | en |
| Orcid | Farhad Safarpoor Dehkordi [0000-0002-1921-1348] | en |
| Issued Date | 2019-05-31 | en |
| Abstract | Background: Regarding the presence of immunosuppressed patients in hospitals, hospital food must have a boost safety. Escherichia coli O157 is one of the prevalent causes of food-related poisoning. Objectives: The current examination was done to assess the distribution of virulence factors and phenotypic analysis of antibiotic resistance of E. coli O157 bacteria recovered from hospital food. Methods: From April to August 2016, 200 hospital food samples were obtained and directly transported to the laboratory. Escherichia coli O157-positive bacteria were analyzed by disk diffusion and polymerase chain reaction (PCR). Results: Nine out of 200 (4.50%) samples harbored E. coli O157. Distribution of E. coli O157 in soup and gavage samples were 3% and 6%, respectively. Stx1 (100%), eaeA (100%), and ehlyA (100%) were the most frequently detected virulence genes. Escherichia coli O157 bacteria exhibited maximum prevalence of antibiotic resistance against tetracycline (100%), gentamycin (100%), ampicillin (100%), mezlocillin (50%), enrofloxacin (50%), and trimethoprim (50%). Distribution of resistance of E. coli O157 bacteria against more than six antibiotic agents was 11.11%. Conclusions: Gavage and soup samples may be sources of virulent and resistant E. coli O157. High presence of E. coli O157, simultaneous presence of multiple virulence genes, and resistance against animal-based antibiotics presented inadequacy of cooking time and temperature in processing of hospital foods. | en |
| DOI | https://doi.org/10.5812/jjm.82659 | en |
| Keyword | Virulence Factors | en |
| Keyword | Antibiotic Resistance Properties | en |
| Keyword | Hospital Food | en |
| Keyword | <i>Escherichia coli</i> O157 | en |
| Publisher | Brieflands | en |
| Title | Prevalence of Antibiotic Resistance and Distribution of Virulence Factors in the Shiga Toxigenic <i>Escherichia coli</i> Recovered from Hospital Food | en |
| Type | Research Article | en |
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