A Novel Reduction Strategy of Clarithromycin Resistance in Helicobacter pylori
| Author | Omid Tadjrobehkar | en |
| Author | Hamid Abdollahi | en |
| Issued Date | 2014-12-01 | en |
| Abstract | Background: Antibiotic resistance is a major therapeutic problem in patients infected with Helicobacter pylori. H. pylori clarithromycin resistant mutants have been evolved during antibiotic therapy, this is mainly due to 23s rRNA point mutations. Objectives: In the present study, we investigated anti-mutational features of four traditionally Iranian medicinal plants on three local isolated H. pylori strains. Materials and Methods: In this study clarithromycin resistance was used as a mutation indicator. Frequencies of such mutations in the presence and absence of plant extracts were evaluated. Mutation incidence was evaluated by Luria Delbruck fluctuation assay. Results: The mean mutation frequency in H. pylori isolates was 27 × 10-9 which decreased at the presence of Mirtus communis, Teucrium polium, Achillea millefolium and Thymus vulgaris of plant extract, this amount was 97.4%, 95.2%, 63.7% and 19.6% respectively. Moreover, A-to-G transition at 2143 position (A2143G) was detected by PCR-sequencing as major point mutation causing clarithromycin resistant mutants. Conclusions: The efficacy of these plant extracts in prohibiting resistance showed considerable results. This finding should be considered to use plant extracts with antibiotics to develop more effective eradication regimens. | en |
| DOI | https://doi.org/10.5812/jjm.13081 | en |
| Keyword | Medicinal Plants | en |
| Keyword | <i>Helicobacter pylori</i> | en |
| Keyword | <i>Achillea millefolium</i> | en |
| Keyword | <i>Teucrium polium</i> | en |
| Keyword | <i>Mirtus communis</i> | en |
| Keyword | Clarithromycin | en |
| Keyword | Mutation | en |
| Publisher | Brieflands | en |
| Title | A Novel Reduction Strategy of Clarithromycin Resistance in Helicobacter pylori | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- 18790-pdf.pdf
- Size:
- 102.85 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF