Advantage of Applying OSC to 1H NMR-Based Metabonomic Data of Celiac Disease
| Author | Mostafa Rezaei-Tavirani | en |
| Author | Fariba Fathi | en |
| Author | Fatemeh Darvizeh | en |
| Author | Mohamad Reza Reza Zali | en |
| Author | Mohamad Rostami Rostami Nejad | en |
| Author | Kamran Rostami | en |
| Author | Mohsen Tafazzoli | en |
| Author | Afsaneh Arefi Arefi oskouie | en |
| Author | Seyed AbdolReza AbdolReza Mortazavi-Tabatabaei | en |
| Issued Date | 2012-06-30 | en |
| Abstract | Background: Celiac disease (CD) is a disorder associated with body reaction to gluten. After the gluten intake, an immune reaction against the protein occurs and damages villi of small intestine in celiac patients gradually. Objectives: The OSC, a filtering method for minimization of inter- and intra-spectrometer variations that influence on data acquisition, was applied to biofluid NMR data of CD patients. Patients and Methods: In this study, metabolites of total 56 serum samples from 12 CD patients, 15 CD patients taking gluten-free diet (GFD), and 29 healthy cases were analyzed using nuclear magnetic resonance (NMR) and associated theoretical analysis. Employing ProMetab (version ProMetab_v3_3) software, data obtained from NMR spectra were reduced and orthogonal signal correction (OSC) effect on celiac disease metabonomics before and after the separation by principle component analysis (PCA) was investigated. Results: The three groups were separated by OSC and findings were analyzed by partial least squares discriminant analysis (PLS-DA) method. Root mean square error of calibration (RMSEc) and correlation coefficient of calibration (Rc) for PLS-DA referred to an efficient group separation filtered by OSC. Conclusions: The applied leave-one-out cross-validation to PLS-DA method performed along with OSC confirmed validation of data analysis. Finally four metabolites are introduced as CD biomarkers. | en |
| DOI | https://doi.org/10.5812/ijem.3058 | en |
| URI | https://brieflands.com/journals/ijem/articles/77506 | en |
| Keyword | Magnetic Resonance Spectroscopy | en |
| Keyword | Principle Component Analysis | en |
| Keyword | Discriminant Analysis | en |
| Keyword | Celiac Disease | en |
| Publisher | Brieflands | en |
| Title | Advantage of Applying OSC to <sup>1</sup>H NMR-Based Metabonomic Data of Celiac Disease | en |
| Type | Research Article | en |
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