A Prospective to Regulatory Role of miRNAs on Wnt/β-catenin Signaling and Its Crosstalk to the Other Cellular Pathways in Tumorigenesis of Glioblastoma by a Systems Biology Approach
| Author | Morteza Saeidi | en |
| Author | Alireza Pasdar | en |
| Author | Farzad Rahmani | en |
| Author | Abozar Ghorbani | en |
| Author | Negar Mottaghi | en |
| Author | Forouzan Amerizadeh | en |
| Orcid | Morteza Saeidi [0000-0002-9222-3660] | en |
| Orcid | Alireza Pasdar [0000-0002-7864-9729] | en |
| Orcid | Abozar Ghorbani [0000-0002-2183-0033] | en |
| Orcid | Forouzan Amerizadeh [0000-0003-4213-1984] | en |
| Issued Date | 2025-12-31 | en |
| Abstract | Background: The Wnt plays a crucial role in the initiation, progression, and spread of glioblastoma (GBM). Recently, microRNAs (miRNAs) have been demonstrated to be key players in controlling cell growth and tumor formation. Objectives: The present study offers the latest insight into miRNAs that influence the Wnt pathway and their interaction with protein-coding genes. Methods: Previous studies on the regulatory function of miRNAs targeting the Wnt/catenin pathway were reviewed, and all miRNA-targeted genes were found in the miRDB database. Protein-protein interactions (PPIs) of miRNA-targeted genes were investigated using String and Cytoscape software, and hub proteins were examined. Gene-subnetwork Gene Ontology (GO) analysis was performed. Results: At first, 13 downregulated and 25 upregulated miRNAs targeting the Wnt pathway were obtained, each targeting 1,685 and 1,313 genes, respectively; 12 and 15 hub proteins were found in dysregulated miRNA-targeted genes, which interacted with most genes. The PPI network analysis and subnetwork GO analysis showed these proteins cross-talk with many other proteins that have key roles in the pathways that cause proliferation and malignancy in cells. Conclusions: Hub proteins are oncogenic proteins that increase gene replication and suppress apoptotic pathways, or tumor suppressors that prevent cancer. By focusing on hub proteins alone or as part of a multi-target approach, it is possible to treat GBM tumors successfully. | en |
| DOI | https://doi.org/10.5812/ijcm-156834 | en |
| URI | https://brieflands.com/journals/ijcm/articles/156834 | en |
| Keyword | Glioblastoma | en |
| Keyword | Wnt/β-catenin Signaling | en |
| Keyword | Systems Biology | en |
| Keyword | Protein-Protein Interaction | en |
| Keyword | Subnetwork Analysis | en |
| Publisher | Brieflands | en |
| Title | A Prospective to Regulatory Role of miRNAs on Wnt/β-catenin Signaling and Its Crosstalk to the Other Cellular Pathways in Tumorigenesis of Glioblastoma by a Systems Biology Approach | en |
| Type | Research Article | en |